An inverted repeat in the Tn21 integron determines the production of stem-loop DNA

A 580-bp-long stem-loop DNA (sl-DNA) molecule was produced by a recombinant plasmid containing complete int and aadA genes from the transposon Tn21 cloned in the vector pUC18. A 50-bp-long inverted repeat found near the int gene stop codon could be involved in the generation of the sl-DNA molecule....

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Bibliographic Details
Published in:Plasmid 1996-01, Vol.35 (1), p.31-36
Main Authors: Francia, M V, García Lobo, J M
Format: Article
Language:English
Subjects:
Codon - genetics
DNA Replication
DNA Transposable Elements - genetics
DNA, Bacterial - genetics
DNA, Satellite - genetics
Nucleic Acid Conformation
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
Trimethoprim Resistance - genetics
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Summary:A 580-bp-long stem-loop DNA (sl-DNA) molecule was produced by a recombinant plasmid containing complete int and aadA genes from the transposon Tn21 cloned in the vector pUC18. A 50-bp-long inverted repeat found near the int gene stop codon could be involved in the generation of the sl-DNA molecule. cloning of the dhfrII gene between the plasmid replication origin and this IR produced an extended sl-DNA including the dhfrII gene. Tn21 recombination hot spot 2 was also able to direct the production of sl-DNA.
ISSN:0147-619X
DOI:10.1006/plas.1996.0003