Development of protamine-bonded phase for separation of saccharides in liquid chromatography

A protamine-bonded polymer gel was synthesized by passing this protein through an activated carbamate polyamine polymer gel layer. This new packing material was suitable for versatile separation of monosaccharides, oligosaccharides, sugar alcohols and uronic acids in liquid chromatography. The colum...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1996-06, Vol.737 (2), p.149-156
Main Authors: Yamauchi, Shuji, Hanai, Toshihiko, Suzuki, Junko, Ito, Masae, Sano, Yukiko, Shibata, Ryoko, Kinoshita, Toshio, Yaginuma, Masakimi, Kadowaki, Kazuomi, Takahashi, Yuichiro
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Carbohydrates - isolation & purification
Carbohydrates - urine
Chromatography, Gel - methods
Chromatography, High Pressure Liquid - methods
Humans
Molecular Sequence Data
Protamine
Protamines - chemistry
Saccharides
Spectrometry, Fluorescence
Stationary phases, LC
Sugar alcohols
Uronic acids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A protamine-bonded polymer gel was synthesized by passing this protein through an activated carbamate polyamine polymer gel layer. This new packing material was suitable for versatile separation of monosaccharides, oligosaccharides, sugar alcohols and uronic acids in liquid chromatography. The column packed with this gel could be operated at room temperature and gave excellent recovery for the reducing monosaccharides such as 2-deoxyribose, 2-deoxyglucose, ribose and mannose for which the conventional alkylamine-bonded phase column gave poor recovery. This column was used for the analysis of saccharides in urine using a post-column reaction detection system.
ISSN:0021-9673
DOI:10.1016/0021-9673(96)00012-X