Presence of lipids in urinary stones: results of preliminary studies

The presence of lipids in urinary stones was determined by histochemical and biochemical methods. When crystals of calcium oxalate, made by mixing calcium chloride and potassium oxalate solutions and sections of human calcium oxalate urinary stones, were exposed to osmium vapors, there was no staini...

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Bibliographic Details
Published in:Calcified tissue international 1988-02, Vol.42 (2), p.91-96
Main Authors: KHAN, S. R, SHEVOCK, P. N, HACKETT, R. L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcium Oxalate - analysis
Histocytochemistry - methods
Humans
Magnesium - analysis
Magnesium Compounds
Medical sciences
Microscopy, Electron, Scanning
Nephrology. Urinary tract diseases
Phosphates - analysis
Phospholipids - analysis
Struvite
Uric Acid - analysis
Urinary Calculi - analysis
Urinary lithiasis
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Summary:The presence of lipids in urinary stones was determined by histochemical and biochemical methods. When crystals of calcium oxalate, made by mixing calcium chloride and potassium oxalate solutions and sections of human calcium oxalate urinary stones, were exposed to osmium vapors, there was no staining of the pure crystals whereas the stone sections were stained. De-paraffinized sections of demineralized calcium oxalate stones showed positive sudanophilia on staining with Sudan black B. Both these experiments indicate the presence of lipids in calcium oxalate stones. Lipids were extracted from uric acid, struvite, and calcium oxalate stones using standard techniques. Phospholipids were separated by one-dimensional thin layer chromatography. All the stones studied contained lipids. In calcium oxalate stones they accounted for 10.15% of the matrix. Calcium oxalate and struvite stones contained more phospholipids than uric acid stones. Cardiolipin, sphingomyelin, phosphatidyl choline, phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl glycerol were identified in lipid extracts. Demineralization by ethylenediaminetetra-acetate (EDTA) treatment increased lipid output from calcium oxalate stones by 15.5%.
ISSN:0171-967X
1432-0827
DOI:10.1007/BF02556340