Physical map of the western X-disease phytoplasma chromosome

A physical map of the chromosome of the western X-disease phytoplasma was constructed and represents the first physical map of a phytoplasma chromosome. The western X-disease phytoplasma is a nonculturable, plant-pathogenic member of the class Mollicutes and is the causal agent of a severe disease o...

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Bibliographic Details
Published in:Journal of Bacteriology 1996-07, Vol.178 (13), p.3985-3988
Main Authors: Firrao, G. (Universita di Udine, Udine, Italy.), Smart, C.D, Kirkpatrick, B.C
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
BACTERIA
Bacteriology
BACTERIOSE
BACTERIOSIS
Blotting, Southern
CHROMOSOME
Chromosomes, Bacterial
CROMOSOMAS
Electrophoresis, Gel, Pulsed-Field
Fruit - microbiology
Genes
GENETICA
GENETIQUE
Mollicutes
ORGANISMOS PATOGENOS
Plant Diseases - microbiology
Restriction Mapping
Tenericutes - genetics
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Summary:A physical map of the chromosome of the western X-disease phytoplasma was constructed and represents the first physical map of a phytoplasma chromosome. The western X-disease phytoplasma is a nonculturable, plant-pathogenic member of the class Mollicutes and is the causal agent of a severe disease of fruit trees in North America. The map was generated by performing restriction digests of the chromosome and resolving the restriction fragments by pulsed-field gel electrophoresis. Southern blot analysis using cloned phytoplasma probes confirmed the arrangement of contiguous restriction fragments. The locations of 20 restriction sites for the enzymes SalI, XhoI, BssHII, RsrII, SmaI, and NotI were mapped on the chromosome, which is circular and comprises approximately 670 kb. The locations of the two rRNA operons and of four previously cloned fragments of chromosomal DNA were also placed on the map
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.178.13.3985-3988.1996