DOS, a Novel Pleckstrin Homology Domain–Containing Protein Required for Signal Transduction between Sevenless and Ras1 in Drosophila

The specification of the R7 photoreceptor cell in the developing eye of Drosophila is dependent upon activation of the Sevenless (SEV) receptor tyrosine kinase. By screening for mutations that suppress signaling via a constitutively activated SEV protein, we have identified a novel gene, daughter of...

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Bibliographic Details
Published in:Cell 1996-06, Vol.85 (6), p.911-920
Main Authors: Raabe, Thomas, Riesgo–Escovar, Juan, Liu, Xiangdong, Bausenwein, Burkhard S, Deak, Peter, Maröy, Peter, Hafen, Ernst
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Animals, Genetically Modified
Binding Sites
Blood Proteins
Crosses, Genetic
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Eye - chemistry
Eye Proteins - analysis
Eye Proteins - genetics
Eye Proteins - physiology
Female
Genes, Insect - genetics
Genes, Suppressor
Insect Hormones - genetics
Insect Hormones - metabolism
Male
Membrane Glycoproteins - genetics
Membrane Glycoproteins - physiology
Molecular Sequence Data
Mutation
Phosphoproteins
Photoreceptor Cells, Invertebrate - growth & development
ras Proteins - genetics
ras Proteins - physiology
Receptor Protein-Tyrosine Kinases - physiology
Sequence Homology, Amino Acid
Signal Transduction - physiology
Wings, Animal - growth & development
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Summary:The specification of the R7 photoreceptor cell in the developing eye of Drosophila is dependent upon activation of the Sevenless (SEV) receptor tyrosine kinase. By screening for mutations that suppress signaling via a constitutively activated SEV protein, we have identified a novel gene, daughter of sevenless (dos). DOS is required not only for signal transduction via SEV but also in other receptor tyrosine kinase signaling pathways throughout development. The presence of an amino-terminally located pleckstrin homology domain and many potential tyrosine phosphorylation sites suggests that DOS functions as an adaptor protein able to interact with multiple signaling molecules. Our genetic analysis demonstrates that DOS functions upstream of Ras1 and defines a signaling pathway that is independent of direct binding of the DRK SH2/SH3 adaptor protein to the SEV receptor tyrosine kinase.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)81274-X