Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection

The fertilization rates and further development of 528 human metaphase IT oocytes directly injected by a single spermatozoon were analysed with respect to their morphological features at the light microscopy level at the time of retrieval. The deviations of oocyte morphology which were most frequent...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1996-03, Vol.11 (3), p.595-597
Main Authors: Sutter, Paul De, Dozortsev, Dmitri, Qian, Chen, Dhont, Marc
Format: Article
Language:English
Subjects:
Biological and medical sciences
Birth control
Cytoplasm
Embryonic and Fetal Development
Female
Fertilization in Vitro - methods
Gynecology. Andrology. Obstetrics
Humans
intracytoplasmic sperm injection
Male
Medical sciences
Microinjections
oocyte morphology
Oocytes - ultrastructure
Retrospective Studies
Spermatozoa
Sterility. Assisted procreation
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Summary:The fertilization rates and further development of 528 human metaphase IT oocytes directly injected by a single spermatozoon were analysed with respect to their morphological features at the light microscopy level at the time of retrieval. The deviations of oocyte morphology which were most frequently observed, after removal of cumulus cells, were dark incorporations, dark zona pellucida, large peri-vitelline space, spots, vacuoles, refractile bodies and irregular shape. These deviations correlated neither with the fertilization rate nor with the embryo quality score, as compared to ‘ideal’ oocytes. Since the majority of oocytes displayed deviations from the ‘ideal’ morphotype but were still fertilized and developed in culture at a normal rate, they were probably as normal as ‘ideal’ oocytes. Since some of these morphotypes, such as refractile bodies, have been shown to be associated with failure of fertilization, it seems that intracytoplasmic sperm injection may be an appropriate method of treatment for couples in whom repeated failure of in-vitro fertilization is associated with the retrieval of dysmorphic oocytes in the presence of normal semen characteristics.
ISSN:0268-1161
1460-2350
DOI:10.1093/HUMREP/11.3.595