Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated mol...

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Bibliographic Details
Published in:Parasitology 1996-07, Vol.113 (1), p.63-69
Main Authors: De Graaf, D. C., Berghen, P., Moens, L., De Marez, T. M., Raes, S., Blaxter, M. L., Vercruysse, J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Helminth - biosynthesis
Antigens, Helminth - chemistry
Antigens, Helminth - immunology
Antigens, Helminth - isolation & purification
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Blotting, Western
Cattle
Chromatography, Gel
Chromatography, Ion Exchange
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Globins - chemistry
Globins - immunology
Globins - isolation & purification
haemoglobin
Immune Sera - immunology
Invertebrates
Molecular Sequence Data
Molecular Weight
Nemathelminthia. Plathelmintha
nematode
Nippostrongylus - immunology
Ostertagia - immunology
Ostertagia ostertagi
Physiology. Development
Rabbits
Sequence Alignment
Trichostrongylus - immunology
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Summary:Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000066282