Methylation of the polyomavirus major capsid protein VP1

Polyomavirus VP1 has been shown to be modified by phosphorylation, sulfation, acetylation and hydroxylation. The major capsid protein VP1 is now shown to be modified by methylation. Addition of cycloheximide to infected cultures followed by addition of [ 3H-methyl]- l-methionine and subsequent immun...

Full description

Saved in:
Bibliographic Details
Published in:Virus research 1996-02, Vol.40 (2), p.141-147
Main Authors: Burton, Kenneth S., Consigli, Richard A.
Format: Article
Language:English
Subjects:
Animals
Capsid - metabolism
Capsid Proteins
Cells, Cultured
Cycloheximide - pharmacology
Haplorhini
Major capsid protein VP1
Methionine - pharmacology
Methylation
Polyomavirus
Polyomavirus - drug effects
Polyomavirus - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Polyomavirus VP1 has been shown to be modified by phosphorylation, sulfation, acetylation and hydroxylation. The major capsid protein VP1 is now shown to be modified by methylation. Addition of cycloheximide to infected cultures followed by addition of [ 3H-methyl]- l-methionine and subsequent immunoprecipitation, SDS-PAGE and fluorography revealed methylation occurring on VP1. Amino acid analysis of [ 3H]-methyl]- l-methionine-labelled polyomavirus VP1 by two-dimensional paper chromatography and HPLC of the acid-hydrolyzed protein revealed the presence of 3H-labelled trimethyllysine and monomethylarginine.
ISSN:0168-1702
1872-7492
DOI:10.1016/0168-1702(95)01266-4