Regional expression and subcellular localization of the tyrosine-specific phosphatase SH-PTP2 in the adult human nervous system

The protein tyrosine phosphatase SH-PTP2 has been implicated in a variety of cell signaling cascades, including those mediating neuronal survival. We therefore investigated the expression of SH-PTP2 in the adult human nervous system using Western blotting, immunohistochemistry and immunoelectron mic...

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Bibliographic Details
Published in:Neuroscience 1996-04, Vol.71 (4), p.1037-1042
Main Authors: Reeves, S.A., Ueki, K., Sinha, B., Difiglia, M., Louis, D.N.
Format: Article
Language:English
Subjects:
Adult
Blotting, Western
brain
Brain - cytology
Brain - enzymology
expression
human
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins
Microscopy, Electron
neurons
Neurons - enzymology
Neurons - ultrastructure
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases - analysis
PTPID
SH-PTP2
SH2 Domain-Containing Protein Tyrosine Phosphatases
Spinal Cord - cytology
Spinal Cord - enzymology
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Summary:The protein tyrosine phosphatase SH-PTP2 has been implicated in a variety of cell signaling cascades, including those mediating neuronal survival. We therefore investigated the expression of SH-PTP2 in the adult human nervous system using Western blotting, immunohistochemistry and immunoelectron microscopy. SH-PTP2 immunoreactivity was noted only in neurons, but was not restricted to a specific neuronal type or location. Immunohistochemistry showed perikaryal staining, whereas Western blotting and ultrastructural analysis suggested that SH-PTP2 is present in axons as well. While immunohistochemistry showed a Nissl-like pattern in large motor neurons, immunoelectron microscopy demonstrated a diffuse pattern of cytoplasmic staining, without apparent preferential localization. The presence of the SH2 domain-containing tyrosine-specific phosphatase SH-PTP2 in diverse neurons in the adult nervous system suggests that SH-PTP2 may play a role in a broad spectrum of neuronal responses.
ISSN:0306-4522
1873-7544
DOI:10.1016/0306-4522(95)00491-2