Constitutive expression of specific interferon isotypes in peripheral blood leukocytes from normal individuals and in promonocytic U937 cells

Constitutive expression of IFN‐α5 and IFN‐β was detected in different lymphoid cells including peripheral blood mononuclear cells from normal individuals following amplification of IFN mRNA by reverse transcriptase–polymerase chain reaction and direct sequencing of the amplified product. The activat...

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Bibliographic Details
Published in:Journal of leukocyte biology 1996-07, Vol.60 (1), p.137-146
Main Authors: Lallemand, Christophe, Lebon, Pierre, Rizza, Paola, Blanchard, Brigitte, Tovey, Michael G.
Format: Article
Language:English
Subjects:
Base Sequence
Cell Line
Cell Nucleus - metabolism
Cloning, Molecular
Consensus Sequence
DNA Primers
DNA-Binding Proteins - metabolism
Gene Expression
Genes, MHC Class I
homeostasis
Humans
Interferon-alpha - biosynthesis
Interferon-beta - biosynthesis
Interferon-Stimulated Gene Factor 3
Interferon-Stimulated Gene Factor 3, gamma Subunit
interferon‐α5
interferon‐β
ISGF‐3
Leukocytes - immunology
luciferase
Luciferases - biosynthesis
major histocompatibility complex class I
Molecular Sequence Data
Monocytes - immunology
Polymerase Chain Reaction
Recombinant Proteins - biosynthesis
RNA, Messenger - biosynthesis
Sequence Homology, Nucleic Acid
Transcription Factors - metabolism
Tumor Cells, Cultured
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Summary:Constitutive expression of IFN‐α5 and IFN‐β was detected in different lymphoid cells including peripheral blood mononuclear cells from normal individuals following amplification of IFN mRNA by reverse transcriptase–polymerase chain reaction and direct sequencing of the amplified product. The activated form of the interferon‐induced transcription factor complex ISGF3 was also dectected in nuclear extracts from uninduced cells. Culture supernatants from uninduced U937 cells were also found to activate an ISRE cloned upstream of the luciferase reporter gene, indicating the presence of endogenous IFN activity equivalent to approximately 0.3 to 0.5 IU/mL. This endogenous IFN was also shown to play a role in mamtaining the basal level of expression of the major histocompatibility class I genes in lymphoid cells. These results suggest that IFN‐α5 and IFN‐β are produced at low levels in normal tissues and play an important role in the regulation of cell function and in the maintenance of homeostasis.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.60.1.137