Genetically transformed maize plants from protoplasts

Genetically transformed maize plants were obtained from protoplasts treated with recombinant DNA. Protoplasts that were digested from embryogenic cell suspension cultures of maize inbred A188 were combined with plasmid DNA containing a gene coding for neomycin phosphotransferase (NPT II) next to the...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-04, Vol.240 (4849), p.204-207
Main Authors: Rhodes, C.A, Pierce, D.A, Mettler, I.J, Mascarenhas, D, Detmer, J.J
Format: Article
Language:English
Subjects:
ADN
Agrobacterium tumefaciens
Agronomy. Soil science and plant productions
Biochemistry
Biological and medical sciences
Biotechnology
Callus
Cell growth
Cell lines
Cell Membrane Permeability
Cells, Cultured
Corn
DNA
DNA - analysis
DNA, Recombinant
Electricity
Electroporation
Fruits & vegetables
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic aspects
Genetic engineering
Genetic Engineering - methods
Genetic engineering applications
Genetic technics
GENETIC TRANSFORMATION
Genetics
Genetics and breeding of economic plants
Kanamycin Kinase
Methods. Procedures. Technologies
Phosphotransferases - metabolism
Plant breeding: fundamental aspects and methodology
Plant genetic engineering
Plants
PLASMIDE
PLASMIDIOS
PLASMIDS
Promoter regions
PROTOPLASTE
PROTOPLASTO
PROTOPLASTS
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
Transformation, Genetic
Transformed cell line
Vectors (cloning, transfer, expression). Insertion sequences and transposons
ZEA MAYS
Zea mays - genetics
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Summary:Genetically transformed maize plants were obtained from protoplasts treated with recombinant DNA. Protoplasts that were digested from embryogenic cell suspension cultures of maize inbred A188 were combined with plasmid DNA containing a gene coding for neomycin phosphotransferase (NPT II) next to the 35S promoter region of cauliflower mosaic virus. A high voltage electrical pulse was applied to the protoplasts, which were then grown on filters placed over feeder layers of maize suspension cells (Black Mexican Sweet) and selected for growth in the presence of kanamycin. Selected cell lines showed NPT II activity. Plants were regenerated from transformed cell lines and grown to maturity. Southern analysis of DNA extracted from callus and plants indicated the presence of the NPT II gene.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2832947