Nucleotide sequence of the Australian bluetongue virus serotype 1 RNA segment 10

CSIRO, Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on pu...

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Bibliographic Details
Published in:Journal of general virology 1988-04, Vol.69 (4), p.945-949
Main Author: Gould, A.R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
Base Sequence
Biological and medical sciences
Bluetongue virus
Bluetongue virus - genetics
DNA - genetics
Fundamental and applied biological sciences. Psychology
Genes, Viral
genetic variation
Genetics
Microbiology
Molecular Sequence Data
nucleotide sequences
proteins
Reoviridae - genetics
RNA
RNA, Double-Stranded - genetics
RNA, Viral - genetics
Virology
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Summary:CSIRO, Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on purified double-stranded RNA molecules. The gene segment was 822 nucleotides in length and capable of coding for two proteins on either 229 or 216 amino acids, having net charges of either +4.5 or +5.5 respectively at neutral pH. Comparison with the RNA segment 10 from BTV serotype 10 revealed a high degree of amino acid sequence conservation as well as regions of nucleic acid sequence conservation. Keywords: BTV, genome segment 10, nucleotide sequence Received 23 October 1987; accepted 14 January 1988.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-69-4-945