The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay

A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing “antioxidant power.” Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the...

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Bibliographic Details
Published in:Analytical biochemistry 1996-07, Vol.239 (1), p.70-76
Main Authors: Benzie, Iris F.F., Strain, J.J.
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Antioxidants - analysis
Cattle
Colorimetry - methods
Dose-Response Relationship, Drug
Ferric Compounds - chemistry
Ferrous Compounds - chemistry
Humans
Indicators and Reagents
Kinetics
Middle Aged
Oxidation-Reduction
Plasma - metabolism
Reproducibility of Results
Triazines - chemistry
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Summary:A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing “antioxidant power.” Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, α-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1996.0292