Chromosomal localization of Cdx2, a murine homologue of the Drosophila gene Caudal, to mouse chromosome 5

Members of the homeobox gene family share a conserved sequence of 180 nucleotides encoding 60 amino acids known as the homeodomain. The homeodomain-containing proteins act as transcription factors, which often play significant roles in pattern formation and in determining the positional identity of...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-06, Vol.34 (2), p.270-271
Main Authors: CHAWENGSAKSOPHAK, K, BECK, F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CDX2 Transcription Factor
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Crosses, Genetic
Drosophila - genetics
Drosophila melanogaster
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Genes, Homeobox
Genetics of eukaryotes. Biological and molecular evolution
Homeodomain Proteins - genetics
Humans
Mice
Mice, Inbred C57BL - genetics
Muridae - genetics
Species Specificity
Trans-Activators
Transcription Factors
Vertebrata
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Summary:Members of the homeobox gene family share a conserved sequence of 180 nucleotides encoding 60 amino acids known as the homeodomain. The homeodomain-containing proteins act as transcription factors, which often play significant roles in pattern formation and in determining the positional identity of the cells during development. Drosophila caudal is a homeobox-containing gene first described by Mlodzik. Three caudal homologues in mouse have recently been characterized: Cdx1 (2), Cdx2, and Cdx4. These three genes exhibit some overlapping as well as unique tissue-specific expression during embryogenesis. Interestingly, expression in the embryonic gut is conserved in both Drosophila caudal and its mouse homologues: Cdx1, Cdx2, and Cdx4. Cdx1 is located on mouse chromosome 18. Recently, Cdx4 has been located on the mouse X chromosome but Cdx2 has not been located. Here we report the localization of Cdx2 in the mouse genome using interspecific backcross mapping. A DNA panel from interspecific backcrossed mice (C57BL/6J x Mus spretus)F1 x Mus spretus (BSS) was obtained from the Jackson Laboratory. Restriction fragment length polymorphisms (RFLPs) between the two parental strains were identified by Southern analysis using various restriction enzymes and a Cdx2-specific probe (a BamHI to StyI fragment of 400 bp). Polymorphic bands of 2.3 and 3.8 kb were detected between Mus spretus and C57BL/6J, respectively, with the restriction enzyme BamHI. Additional polymorphisms were seen with HindIII (12 and 17 kb) and with BglII (8 and 13 kb). DNA from 94 backcross animals was typed. The segregation pattern of the backcross animals was sent to the Jackson Laboratory to compare to that of known markers. Cdx2 is linked to a number of loci assigned to the distal end of mouse chromosome 5. These loci are D5Mit9 (The Jackson Laboratory Backcross public database, accessible on the World Wide Web at http:/www.jax.org/resources/documents/cmdata), nitric oxide synthase (Nos1), pancreatic and duodenal homeobox gene 1 (Pdx1), previously known as insulin promoter factor 1 (Ipf1), polytropic murine leukemia virus 12 (Pmv12), and cytoskeletal beta actin (Actb). The most probable order of these loci is D5Mit9, Nos1, (Pdx1, Pmv12, Cdx2), and Actb. The location of human CDX2 has not been directly assigned. However, human CDX3 was localized to chromosome 13q12.3 using fluorescence in situ hybridization with a Cdx3 probe. Cdx3 in hamster is orthologous to Cdx2 in mouse, suggesting that human
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0286