Characterization of four microsatellites in an Italian population and their application to paternity testing

Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been...

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Bibliographic Details
Published in:Molecular and cellular probes 1996-04, Vol.10 (2), p.155-158
Main Authors: Sirchia, S.M., Garagiola, I., De Andreis, C., Gazzoli, I., Gramegna, M., Colucci, G.
Format: Article
Language:English
Subjects:
Alleles
Child
Dinucleotide Repeats
DNA polymorphism
DNA Primers
DNA, Satellite - blood
DNA, Satellite - genetics
European Continental Ancestry Group - genetics
Female
Genetic Carrier Screening
Genetic Markers
Humans
Italian population
Italy
Male
microsatellites
Paternity
paternity testing
PCR
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
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Summary:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After amplification with primers specific for each locus, alleles were separated and detected by denaturing gel electrophoresis and ethidium bromide staining. High heterozygosity and PIC values were observed for all microsatellites in accordance with data in other Caucasian populations. However, different allele distributions for D8S85, D8S88 and D5S346, due to the presence of additional bands or to different frequencies, were found. D7S460, which has never been fully characterized before, appeared to have five alleles in the range 172 to 188 bp. When used for paternity testing, all microsatellites gave results which were consistent with those obtained with established markers, including apo B 3′HVR, D1S80 and COL2A1. This indicates that D8S85, D8S88, D5S346 and D7S460 may be useful as additional informative markers or for solving discrepancies in selected cases.
ISSN:0890-8508
1096-1194
DOI:10.1006/mcpr.1996.0021