Photoconversion of some fluorescent markers to a diaminobenzidine product

Retinal whole mounts, brain sections, and astrocyte cultures were labeled with various fluorescent markers. Tissues or cells were then irradiated by light in the presence of diaminobenzidine. Irradiation initiated a reaction in which specific fluorescent labeling was replaced by an insoluble diamino...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1988-05, Vol.36 (5), p.555-559
Main Authors: Sandell, JH, Masland, RH
Format: Article
Language:English
Subjects:
Animals
Astrocytes - cytology
Biological and medical sciences
Cerebellum - cytology
Diverse techniques
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Immunohistochemistry - methods
Molecular and cellular biology
Photochemistry
Rabbits
Rats
Retina - cytology
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Description
Summary:Retinal whole mounts, brain sections, and astrocyte cultures were labeled with various fluorescent markers. Tissues or cells were then irradiated by light in the presence of diaminobenzidine. Irradiation initiated a reaction in which specific fluorescent labeling was replaced by an insoluble diaminobenzidine product. The diaminobenzidine product is more stable than the original fluorescent labeling and can be processed for electron microscopy. In some cases, the reaction product reveals cellular detail that cannot be resolved in the fluorescent labeling. The 10 fluorescent markers tested have widely differing structures, span a broad range of wavelengths, and label several different cellular elements. The photoconversion reaction was successful with all markers and tissues tested.
ISSN:0022-1554
1551-5044
DOI:10.1177/36.5.3356898