Evaluation of a DNA Probe for Identifying Salmonella typhi in Peruvian and Indonesian Bacterial Isolates

The authors previously reported the construction of a DNA probe that recognizes DNA coding for production of Vi capsular antigen. The present study was undertaken to determine the sensitivity and specificity of this DNA probe, using in situ colony hybridization studies, to detect S. typhi among gram...

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Bibliographic Details
Published in:The Journal of infectious diseases 1988-05, Vol.157 (5), p.1051-1053
Main Authors: Rubin, F. A., Kopecko, D. J., Sack, R. B., Sudarmono, P., Yi, A., Maurta, D., Meza, R., Moechtar, M. A., Edman, D. C., Hoffman, S. L.
Format: Article
Language:English
Subjects:
Antigens
Antigens, Viral - genetics
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteriology
Biological and medical sciences
Blood
Cellulose nitrate
Collodion
Concise Clinical and Laboratory Communications
DNA probes
DNA, Bacterial - analysis
DNA, Recombinant
Epidemiology
False Negative Reactions
Filtration - instrumentation
Fundamental and applied biological sciences. Psychology
Gram-Negative Bacteria - genetics
Human bacterial diseases
Humans
Indonesia
Infectious diseases
Medical research
Medical sciences
Microbiology
Nucleic Acid Hybridization
Peru
Salmonella typhi
Salmonella typhi - genetics
Salmonella typhi - isolation & purification
Specimens
Tropical medicine
Typhoid
Typhoid fever
Typhoid Fever - microbiology
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Summary:The authors previously reported the construction of a DNA probe that recognizes DNA coding for production of Vi capsular antigen. The present study was undertaken to determine the sensitivity and specificity of this DNA probe, using in situ colony hybridization studies, to detect S. typhi among gram-negative bacteria freshly isolated from patients from two geographically distant areas. Initial studies conducted in Lima, Peru, were used to establish the conditions used in the subsequent study in Jakarta, Indonesia.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/157.5.1051