A 4.5-Megabase YAC Contig and Physical Map over the Hemochromatosis Gene Region

We have constructed a yeast artificial chromosome (YAC) contig over the candidate hemochromatosis gene region. This contig comprises 16 YACs from the CEPH, Washington University, and ICI YAC libraries and covers 4.5 Mb at 6p21.3–6p22. The complete contig has been restriction mapped, enabling the pre...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-04, Vol.33 (2), p.153-158
Main Authors: Burt, M.J., Smit, D.J., Pyper, W.R., Powell, L.W., Jazwinska, E.C.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosome Walking
Chromosomes, Artificial, Yeast
DNA Primers
Errors of metabolism
Genetic Markers
Hemochromatosis - genetics
Humans
Medical sciences
Metabolic diseases
Miscellaneous hereditary metabolic disorders
Molecular Sequence Data
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Summary:We have constructed a yeast artificial chromosome (YAC) contig over the candidate hemochromatosis gene region. This contig comprises 16 YACs from the CEPH, Washington University, and ICI YAC libraries and covers 4.5 Mb at 6p21.3–6p22. The complete contig has been restriction mapped, enabling the precise relationship between the YACs to be determined and the mapping of a total of 12 STSs. Nine of these are highly polymorphic STSs that are closely linked to hemochromatosis; this series includes D6S265 and D6S1260, which comprise the most proximal and distal markers linked to HC. This is the first YAC contig that spans the hemochromatosis candidate region, and it provides valuable resource material for the cloning of this and other genes in the region distal to the MHC class I complex.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0178