Rapid Detection of Mitochondrial Sequence Polymorphisms Using Multiplex Solid-Phase Fluorescent Minisequencing

This work describes a novel method, multiplex solid-phase fluorescent minisequencing, for the simultaneous detection of several point mutations and /or small deletions and insertions. The method is applied to the analysis of mitochondrial DNA polymorphisms for the purposes of individual identificati...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-05, Vol.34 (1), p.107-113
Main Authors: Tully, Gillian, Sullivan, Kevin M., Nixon, Paula, Stones, Rebecca E., Gill, Peter
Format: Article
Language:English
Subjects:
African Continental Ancestry Group - genetics
Base Sequence
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA Primers
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
European Continental Ancestry Group - genetics
Fluorescence
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genetics, Population
Haplotypes
Human
Humans
Molecular Sequence Data
Polymorphism, Genetic
Probability
Sequence Analysis, DNA - methods
United Kingdom - ethnology
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Description
Summary:This work describes a novel method, multiplex solid-phase fluorescent minisequencing, for the simultaneous detection of several point mutations and /or small deletions and insertions. The method is applied to the analysis of mitochondrial DNA polymorphisms for the purposes of individual identification. A database of 152 British Caucasians and 103 British Afro-Caribbeans has been constructed, and the probability of a chance match between two unrelated individuals is calculated as 0.054 for Caucasians and 0.026 for Afro-Caribbeans.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0247