The structure of anchorin CII, a collagen binding protein isolated from chondrocyte membrane

cDNA clones for anchorin CII (Mr = 34,000), a collagen-binding protein, were isolated from a lambda gt 11 cDNA library prepared from chick cartilage mRNA. Several overlapping clones were characterized which gave rise to an open reading frame coding for 329 residues and a 3'-untranslated segment...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-04, Vol.263 (12), p.5921-5925
Main Authors: Pilar Fernandez, M, Selmin, O, Martin, G R, Yamada, Y, Pfäffle, M, Deutzmann, R, Mollenhauer, J, von der Mark, K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
anchorin CII
Animals
Base Sequence
Binding and carrier proteins
Biological and medical sciences
Carrier Proteins
Cartilage - analysis
cDNA
Chickens
chondrocytes
Collagen - metabolism
DNA - isolation & purification
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Immunosorbent Techniques
Molecular Sequence Data
Nucleic Acid Hybridization
Peptide Fragments
Protein Biosynthesis
Proteins
Rabbits
Receptors, Cell Surface - genetics
Receptors, Collagen
RNA, Messenger - genetics
Trypsin
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Summary:cDNA clones for anchorin CII (Mr = 34,000), a collagen-binding protein, were isolated from a lambda gt 11 cDNA library prepared from chick cartilage mRNA. Several overlapping clones were characterized which gave rise to an open reading frame coding for 329 residues and a 3'-untranslated segment of 500 base pairs. The clones were identified as coding for anchorin by hybrid select translation analysis and by comparing the deduced amino acid sequence with the sequence of 10 tryptic peptides of the protein. A hydrophobic domain of 25 residues interrupted with 3 polar residues was identified with the carboxyl-terminal portion. There was no evidence for an aminoterminal signal peptide. Northern analysis revealed that the 5' probe hybridizes to a single 1.7-kilobases (kb) mRNA species, whereas the 3' probe hybridizes to two mRNA species of 1.7 kb and 5 kb, which are present in many cells including chondrocytes, crop cells, and fibroblasts. The level of anchorin mRNA in chick embryo fibroblasts was increased by infection with Rous sarcoma virus.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)60653-2