Uptake of L-leucine by trout red blood cells and peripheral lymphocytes

The uptake of l-leucine by trout red blood cells and peripheral lymphocytes has been analyzed. The present study shows two functionally different Na+-independent systems for apolar branched-chain amino acids. They are designated as L systems because they share some properties with the mammalian L sy...

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Bibliographic Details
Published in:The Journal of membrane biology 1996-07, Vol.152 (1), p.57-63
Main Authors: Gallardo, M A, Albi, J L, Sánchez, J
Format: Article
Language:English
Subjects:
Animals
Carrier Proteins - metabolism
Erythrocytes - metabolism
Hydrogen-Ion Concentration
Leucine - pharmacokinetics
Lymphocytes - metabolism
Trout - blood
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Summary:The uptake of l-leucine by trout red blood cells and peripheral lymphocytes has been analyzed. The present study shows two functionally different Na+-independent systems for apolar branched-chain amino acids. They are designated as L systems because they share some properties with the mammalian L system. The carrier present in red blood cells has low Km values, is trans-stimulable and not stereospecific for leucine uptake; on the other hand, the system present in lymphocytes is stereospecific for leucine uptake and trans-inhibitable. Both carriers are pH sensitive in a similar fashion at low pHs, but there are important differences at higher pH values (above neutrality). These properties are compared with these of the asc systems previously reported in these cells.
ISSN:0022-2631
1432-1424
DOI:10.1007/s002329900085