Membrane Depolarization and Carbamoylcholine Stimulate Phosphatidylinositol Turnover in Intact Nerve Terminals

Synaptosomes, purified from rat cerebral cortex, were prelabeled with [3H]inositol to study phosphatidylinositol turnover in nerve terminals. Labeled synaptosomes were either depolarized with 40 mM K+ or exposed to carbamoylcholine (carbachol). K+ depolarization increased the level of inositol phosp...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-04, Vol.85 (8), p.2859-2863
Main Authors: Sylvie M. P. Audigier, James K. T. Wang, Greengard, Paul
Format: Article
Language:English
Subjects:
Animals
Atropine
Atropine - pharmacology
Biochemistry and metabolism
Biological and medical sciences
Buffer storage
Calcium - physiology
Carbachol - physiology
carbamoylcholine
Central nervous system
Cerebral Cortex - metabolism
cortex
Depolarization
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Inositol phosphates
Inositol Phosphates - metabolism
Inositols
Male
membrane potential
Membrane Potentials
Muscarinic receptors
Nerves
Phosphatidylinositols
Phosphatidylinositols - metabolism
phosphoinositides
Phospholipids
Pirenzepine - pharmacology
Potassium - pharmacology
Rats
Receptors, Muscarinic - physiology
Sugar Phosphates - metabolism
Synaptosomes
Synaptosomes - metabolism
Time Factors
Vertebrates: nervous system and sense organs
Citations: Items that cite this one
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Description
Summary:Synaptosomes, purified from rat cerebral cortex, were prelabeled with [3H]inositol to study phosphatidylinositol turnover in nerve terminals. Labeled synaptosomes were either depolarized with 40 mM K+ or exposed to carbamoylcholine (carbachol). K+ depolarization increased the level of inositol phosphates in a time-dependent manner. The inositol trisphosphate concentration increased rapidly and transiently, reaching maximum (250% of control) in
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.8.2859