The effect of tetraethylammonium on intracellular calcium concentration in Alzheimer's disease fibroblasts with APP, S182 and E5-1 missense mutations

It has been proposed that the lack of intracellular calcium concentration ([Ca 2+] i) increase induced by the potassium channel blocker tetraethylammonium (TEA) in skin fibroblast cell lines identifies patients with both sporadic and familial Alzheimer's disease (AD). In order to verify this hy...

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Bibliographic Details
Published in:Neuroscience letters 1996-04, Vol.208 (3), p.216-218
Main Authors: Failli, Paola, Tesco, Giuseppina, Ruocco, Carlo, Ginestroni, Andrea, Amaducci, Luigi, Giotti, Alberto, Sorbi, Sandro
Format: Article
Language:English
Subjects:
Aged
Alzheimer Disease - genetics
Alzheimer Disease - metabolism
Alzheimer's disease
Calcium - metabolism
Cell Line
Female
Fibroblasts - drug effects
Humans
Image analysis
Intracellular calcium
Male
Middle Aged
Mutation
Skin fibroblast cell lines
Tetraethylammonium
Tetraethylammonium Compounds - pharmacology
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Summary:It has been proposed that the lack of intracellular calcium concentration ([Ca 2+] i) increase induced by the potassium channel blocker tetraethylammonium (TEA) in skin fibroblast cell lines identifies patients with both sporadic and familial Alzheimer's disease (AD). In order to verify this hypothesis, the effect of TEA on [Ca 2+] i was studied in single fura-2-loaded skin fibroblast cell lines available in the Tissue Bank of the Italian Research Council. Four out of eight familial AD patients (one patient with S182 mutation, one patient with E5-1 mutation and two patients with 717 Val → Ile APP mutation) and two out of five sporadic AD patients showed a positive response to TEA, whereas five out of 11 control lines were unresponsive. Our data suggest that the absence of the TEA-induced increase in [Ca 2+] i in skin fibroblast cell lines does not identify all AD patients.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(96)12586-6