Theoretical Studies of DNA during Gel Electrophoresis

A numerical study of the motion of a long-chain macromolecule in a gel has shown unexpected features. The application of a field appears to induce the chain to contract on itself. This is followed by its ``unwinding'' into an extended configuration. For long chains, the mobility tends towa...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-05, Vol.240 (4854), p.922-924
Main Author: Deutsch, J. M.
Format: Article
Language:English
Subjects:
Analysis
Biochemistry
Biological and medical sciences
Biosynthesis
Chemistry
Chemistry, Physical and theoretical
Disperse state. Micelles
DNA
DNA - isolation & purification
Electric fields
Electrophoresis
Electrophoresis, Agar Gel
Embryos
Ferritins
Five prime untranslated regions
Fundamental and applied biological sciences. Psychology
Gels
Genes
Genetics
Messenger RNA
Molecular biophysics
Molecular dynamics
Nucleic Acid Conformation
Physical chemistry
Physico-chemical properties of biomolecules
Three prime untranslated regions
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Summary:A numerical study of the motion of a long-chain macromolecule in a gel has shown unexpected features. The application of a field appears to induce the chain to contract on itself. This is followed by its ``unwinding'' into an extended configuration. For long chains, the mobility tends toward a constant, in accord with experiments. For the parameter range used, the observed molecular motion differs strongly from assumptions made in the present theory of electrophoresis.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3363374