Up-regulation of cyclooxygenase-2 mRNA in the rat spinal cord following peripheral inflammation

Prostaglandins (PG) have been described as mediators in spinal nociceptive processing after peripheral inflammation. Enzymes essential for PG biosynthesis, cyclooxygenase isozymes COX-1 and COX-2, have not yet been investigated in the spinal cord. In two studies on rats with adjuvant-induced periphe...

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Bibliographic Details
Published in:FEBS letters 1996-07, Vol.390 (2), p.165-169
Main Authors: Beiche, Flora, Scheuerer, Stefan, Brune, Kay, Geisslinger, Gerd, Goppelt-Struebe, Margarete
Format: Article
Language:English
Subjects:
4-morpholinepropanesulfonic acid
Animals
Arthritis, Experimental - enzymology
Arthritis, Experimental - genetics
Base Sequence
basepair
central nervous system
CFA
CNS
complete Freund's adjuvant
COX
cyclooxygenase
Cyclooxygenase 1
Cyclooxygenase 2
DNA Primers - genetics
GAPDH
glyceraldehyde-3-phosphate dehydrogenase
Inflammation
Inflammation - enzymology
Inflammation - genetics
Isoenzymes - genetics
kilobase
Kinetics
Male
Membrane Proteins
Molecular Sequence Data
MOPS
non-steroidal anti-inflammatory drugs
NSAIDs
Polymerase Chain Reaction
prostaglandin
Prostaglandin-Endoperoxide Synthases - genetics
Rats
Rats, Sprague-Dawley
reverse transcription-polymerase chain reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
RT-PCR
saline sodium citrate
Spinal cord
Spinal Cord - metabolism
SSC
Up-Regulation
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Summary:Prostaglandins (PG) have been described as mediators in spinal nociceptive processing after peripheral inflammation. Enzymes essential for PG biosynthesis, cyclooxygenase isozymes COX-1 and COX-2, have not yet been investigated in the spinal cord. In two studies on rats with adjuvant-induced peripheral inflammation levels of mRNA expression of both COX isoforms were analyzed in the lumbar section of the spinal cord using reverse transcription-polymerase chain reaction (RT-PCR) technique. We could show that mRNA of both COX isoforms is expressed constitutively in the spinal cord with COX-2 as the predominant isoform. Six hours after induction of peripheral inflammation, levels of COX-2 mRNA expression were raised significantly in respect to untreated control rats and returned to baseline within 3 days after induction of inflammation. COX-2 might therefore be regarded as the COX isozyme responsible for spinal PG release in nociceptive processing under a peripheral inflammatory stimulus.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00604-7