Adsorption of bovine serum albumin on to titanium powder

The adsorption of bovine serum albumin (BSA) on to titanium powder has been studied as a function of protein concentration and pH, and in the presence of calcium and phosphate ions. Isotherm data have shown that the adsorption process does not follow the Langmuir model (inflection points). The maxim...

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Bibliographic Details
Published in:Biomaterials 1996, Vol.17 (9), p.859-864
Main Authors: Wassell, Diana T.Hughes, Embery, Graham
Format: Article
Language:English
Subjects:
Adsorption
Animals
Biocompatible Materials
Biological and medical sciences
Bovine serum albumin
Calcium
Calcium Phosphates
Cattle
Hydrogen-Ion Concentration
In Vitro Techniques
Materials Testing
Medical sciences
Osmolar Concentration
phosphate
Phosphates
Powders
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Serum Albumin, Bovine
Sodium Acetate
Surface Properties
Technology. Biomaterials. Equipments. Material. Instrumentation
Time Factors
Titanium
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Summary:The adsorption of bovine serum albumin (BSA) on to titanium powder has been studied as a function of protein concentration and pH, and in the presence of calcium and phosphate ions. Isotherm data have shown that the adsorption process does not follow the Langmuir model (inflection points). The maximum adsorption ( Ad max) at pH 6.8 is 1.13 ± 0.21 mg m −2. For the pH dependence of adsorption the amount adsorbed increases with decreasing pH (at pH 5.15 Ad max = 1.31 ± 0.2mg m −2), indicating that hydration effects are important. Adsorption increases and decreases in the presence of calcium (at pH 6.8 for 0.002 m Ad max = 1.73 ± 0.23 mg m −2) and phosphate (at pH 6.8 for 0.002 m Ad max = 0.64 ± 0.14 mgm-2)ions, respectively, indicatingthatelectrostaticeffectsareimportant.Thetimedependence, isotherm and desorption data provide indirect evidence of possible conformational changes in the BSA molecule.
ISSN:0142-9612
1878-5905
DOI:10.1016/0142-9612(96)83280-7