Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications

• Published in: Biosensors & bioelectronics 1996, Vol.11 (9), p.913-922
• Main Authors: Sukhorukov, Gleb B., Montrel, Michael M., Petrov, Alexander I., Shabarchina, Lyudmila I., Sukhorukov, Boris I.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; biosensors; DNA; DNA - analysis; Langmuir-Blodgett film; nucleic acids; polyelectrolyte; self-assembly
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/0956-5663(96)89440-1

Langmiur-Blodgett (LB) and film technologies based on electrostatic attraction self-assembly (SA) are shown to be useful for immobilization of nucleic acids (DNA, polynucleotides) onto solid supports in sensor devices. The nucleic acids were immobilized in complexes with cationic surfactants (for LB) and polycations (for SA). Infrared spectral studies showed that DNA unfolds in multilayer LB films with octadecylamine and conserves its double helical structure in the LB films with dioctadecyldimethylammonium and in the SA films with polyallylamine, polyethylenimine and poly-L-lysine. Atomic groups and the types of interactions determining the complex formation of these films have been identified. The hydration of LB and SA films was studied to find out binding sites of water molecules and to evaluate the flexibility of nucleic acid compounds in the multilayer films. The possibilities of biosensor applications of these LB and SA films were monitored on binding of specific reagents for DNA by DNA-containing films and mononucleotides by a complementary single-stranded polynucleotide immobilized on a positively charged solid support.