Use of a radiolabeled monoclonal antibody against e‐selectin for imaging of endothelial activation in rheumatoid arthritis

• Published in: Arthritis and rheumatism 1996-08, Vol.39 (8), p.1371-1375
• Main Authors: Chapman, Peter T., Jamar, François, Keelan, Edward T. M., Peters, A. Michael, Haskard, Dorian O.
• Format: Article
• Language: English
• Subjects: Aged; Antibodies, Monoclonal - pharmacokinetics; Arthritis, Rheumatoid - diagnostic imaging; Arthritis, Rheumatoid - immunology; Biological and medical sciences; Contrast media. Radiopharmaceuticals; E-Selectin - analysis; E-Selectin - immunology; Endothelium - chemistry; Endothelium - immunology; Female; Humans; Immunoglobulin Fab Fragments - immunology; Immunoglobulins - metabolism; Indium Radioisotopes - pharmacokinetics; Male; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Radionuclide Imaging; Synovitis - diagnostic imaging
• ISSN: 0004-3591; 1529-0131
• DOI: 10.1002/art.1780390815

Objective. To determine the potential of 111Inlabeled anti–E‐selectin monoclonal antibody (MAb) to image localized endothelial activation in rheumatoid arthritis (RA). Methods. Fourteen patients with RA were studied after intravenous administration of 111In‐labeled F(ab′)2 fragments of MAb against the cytokine‐inducible endothelial cell activation antigen E‐selection (MAb 1.2B6). To compare uptake of 1.2B6 with that of nonspecific immunoglobulin 111In‐labeled polyclonal human immunoglobulin (HIG) was separately administered to 6 of these patients and the relative uptake of each tracer was determined. Results. Prominent and discrete uptake of the radiolabeled MAb 1.2B6 was clearly visible in inflamed joints of all patients. Compared with 111In‐HIG, 111In‐1.2B6 provided superior images in terms of sensitivity and image intensity. Furthermore, the distribution of uptake in inflamed joints was different for the 2 tracers, with 1.2B6 showing a more focal localization in synovium. Conclusion. This study demonstrates that it is possible to objectively assess E‐selectin expression on activated endothelium in vivo in patients with RA, using a radiolabeled MAb. This technique has considerable potential for monitoring disease activity and response to therapy in inflammatory diseases.