Urinary 2-Thiothiazolidine-4-Carboxylic Acid (TTCA) as the Major Urinary Marker of Carbon Disulfide Vapor Exposure in Rats

• Published in: Toxicology and industrial health 1996-01, Vol.12 (1), p.81-92
• Main Authors: Cox, Clinton, Que Hee, Shane S., Lynch, Dennis W.
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Azides - urine; Biomarkers - urine; Carbon disulfide; Carbon Disulfide - metabolism; Carbon Disulfide - pharmacokinetics; Collection; Correlation; Dose-Response Relationship, Drug; Gases; Iodine - urine; Male; Males; Markers; Mathematical models; Rats; Rats, Sprague-Dawley; Sulfides - urine; Thiazoles - urine; Thiazolidines; Time Factors; Toxicology; Urine
• ISSN: 0748-2337; 1477-0393
• DOI: 10.1177/074823379601200105

Male Sprague-Dawley rats (200-250 g; 60 per exposure group) were exposed to carbon disulfide (CS2) air concentrations of 0, 50, 150, and 500 ppm(vlv) for 6 hrlday, 5 days/week over six months. Following the exposures, nine rats from each exposure group had four sets of cumulated urines collected (between 0-8, 8-16, 16-24, and 24-48 hr). The urinary parameters measured were: 2-thiothiazolidine-4-carboxylic acid (TTCA), total thioethers (TE), and the compounds responsive to the iodine-azide (IA) test. Urinary TTCA elimination obeyed pseudo-first-order, one-compartment model kinetics of half-time (t0.5) 5.2 +/- 0.3 hr up to 16 hr of collection. The elimination of TE within 16 hr had a t 0.5 of 8.5 +/- 0.6 hr. TTCA, IA, and TE were correlated highly in the first 16 hr. After 16 hr, the to.5 for TE lengthened to 13. 1 hr. At CS2 concentrations of 50, 150, and 500 ppm, the respective t 0.5 for IA-responsive compounds were 12.6, 6.1, and 4.4 hr. TTCA had the highest correlation coefficient and p-valuerelative to CS2 exposure concentration, and also was the most sensitive, precise, and selective urinary marker.