Variations in microsatellite sequences provide evidence for population differences and multiple ribosomal gene repeats within Trichinella pseudospiralis

Enzymatic amplification of expansion segment 5 sequences within domain IV of the large subunit ribosomal DNA generated distinct results among geographical isolates of Trichinella pseudospiralis from Russia, North America, and Australia from both avian and mammalian hosts. Discrete, multiple DNA frag...

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Bibliographic Details
Published in:The Journal of parasitology 1996-08, Vol.82 (4), p.534-538
Main Authors: Zarlenga, D.S. (USDA, ARS, Immunology and Disease Resistance Laboratory, Beltsville, MD.), Aschenbrenner, R.A, Lichtenfels, J.R
Format: Article
Language:English
Subjects:
Animals
Australia
Base Sequence
Biological and medical sciences
Biological taxonomies
Birds
Conserved Sequence
DNA
DNA Primers - chemistry
DNA, Helminth - chemistry
DNA, Ribosomal - chemistry
Electrophoresis, Agar Gel
Female
Fundamental and applied biological sciences. Psychology
Gels
Genetic Variation
Genetics-Evolution
Genomics
Invertebrates
Male
Mammals
Mice
MICROSATELITES
MICROSATELLITE
Microsatellite Repeats
MICROSATELLITES
MOLECULAR SEQUENCE DATA
Nemathelminthia. Plathelmintha
North America
Nucleic acids
NUCLEOTIDE SEQUENCE
Parasites
Parasitology
Polymerase Chain Reaction
Ribosomal DNA
Russia
SECUENCIA NUCLEOTIDICA
Sequence Alignment
SEQUENCE NUCLEOTIDIQUE
Systematics. Geographical distribution
TRICHINELLA
Trichinella - genetics
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Description
Summary:Enzymatic amplification of expansion segment 5 sequences within domain IV of the large subunit ribosomal DNA generated distinct results among geographical isolates of Trichinella pseudospiralis from Russia, North America, and Australia from both avian and mammalian hosts. Discrete, multiple DNA fragments ranging in approximate size from 285 to 360 bp were observed within and among each of the isolates tested. Polymerase chain reaction performed on individual adult parasites from each isolate resulted in multiple DNA fragments that were comparable to those generated from pooled genomic DNA. Sequence analysis of cloned, representative amplified fragments demonstrated that fragment length variation resulted primarily from the dinucleotide (TG)n and trinucleotide (TGC)n microsatellite repeats present within the expansion segment. Results are consistent with both population differences within the species as well as the presence of multiple alleles of the large subunit ribosomal RNA genes within individual parasites
ISSN:0022-3395
1937-2345
DOI:10.2307/3283777