Escherichia coli Secretion of an Active Chimeric Antibody Fragment

A chimeric mouse-human Fab protein that binds specifically to the human carcinoma cell line C3347 has been expressed and secreted from Escherichia coli. This molecule, which contains functionally assembled kappa and Fd proteins, binds as effectively to sites on the surface of C3347 cells as Fab frag...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-05, Vol.240 (4855), p.1041-1043
Main Authors: Better, Marc, Chang, C. Paul, Robinson, Randy R., Horwitz, Arnold H.
Format: Article
Language:English
Subjects:
Active sites (Biochemistry)
Amino Acid Sequence
Animals
Antibodies
Antibodies, immunoglobulins
Antigen-Antibody Complex - immunology
Antigens, Surface - immunology
Base Sequence
Binding sites (Biochemistry)
Biochemistry
Biological and medical sciences
Biotechnology
Cancer
Carcinogens
Carcinoma
Cell Line
Cell lines
Chimera
Disulfides
Dosage
Dose response relationship
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gels
Genes, Immunoglobulin
Genetic aspects
Genetic engineering
Genetic technics
Humans
Immunoglobulin Fab Fragments - genetics
Immunoglobulin Fab Fragments - immunology
Immunoglobulins
Methods. Procedures. Technologies
Mice
Molecular immunology
Molecular Sequence Data
Molecules
Protein research
Proteins
Structure
Transcription, Genetic
Tumors
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Description
Summary:A chimeric mouse-human Fab protein that binds specifically to the human carcinoma cell line C3347 has been expressed and secreted from Escherichia coli. This molecule, which contains functionally assembled kappa and Fd proteins, binds as effectively to sites on the surface of C3347 cells as Fab fragments prepared proteolytically from whole chimeric or mouse antibody. The production in Escherichia coli of foreign heterodimeric protein reagents, such as Fab, should prove useful in the management of human disease.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3285471