Inhibition of glucose uptake by 5-hydroxyindoleacetic acid in the isolated rat soleus muscle

Accumulated end-products were identified to participate in the late development of glucose intolerance and insulin resistance (IR) in patients with chronic renal insufficiency. The possible pathophysiological role of accumulated 5-hydroxy-indoleacetic acid (5HIAA) in the genesis of IR was investigat...

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Bibliographic Details
Published in:International urology and nephrology 1996, Vol.28 (1), p.123-131
Main Authors: Sebeková, K, Spustová, V, Dzúrik, R
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Dose-Response Relationship, Drug
Glucose - metabolism
Hindlimb
Hydroxyindoleacetic Acid - pharmacology
Hypoglycemic Agents - pharmacology
In Vitro Techniques
Insulin - pharmacology
Insulin Resistance
Magnesium - pharmacology
Male
Muscle, Skeletal - metabolism
Rats
Rats, Wistar
Tolbutamide - pharmacology
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Summary:Accumulated end-products were identified to participate in the late development of glucose intolerance and insulin resistance (IR) in patients with chronic renal insufficiency. The possible pathophysiological role of accumulated 5-hydroxy-indoleacetic acid (5HIAA) in the genesis of IR was investigated employing an in vitro animal model. 5HIAA inhibited the basal glucose uptake in isolated rat soleus muscle with intact membrane with A50 = 1.25 mumol/l, and Emax = 88.6%. 5HIAA significantly inhibited the insulin, and tolbutamide stimulated glucose uptake. In Ca and Mg depletion 5HIAA showed a partially additive inhibitory effect, while nonadditive inhibitory activity was observed in the case of K+ excess. It is concluded that 5HIAA is a metabolically active end-product interfering with glucose uptake in muscle at an insulin postreceptor level, and its effect is related to Ca modulation in the insulin regulatory cascade.
ISSN:0301-1623
1573-2584
DOI:10.1007/BF02550149