Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola

Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting f...

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Bibliographic Details
Published in:Current genetics 1996-08, Vol.30 (3), p.251-258
Main Authors: Kema, G.H.J, Verstappen, E.C.P, Todorova, M, Waalwijk, C. (DLO-Research Inst. for Plant Protection (IPO-DLO), Wageningen (Netherlands))
Format: Article
Language:English
Subjects:
Base Sequence
Crosses, Genetic
DNA Primers
EPIDEMIOLOGIA
EPIDEMIOLOGIE
EPIDEMIOLOGY
EPREUVE SUR LA DESCENDANCE
Genetic Markers
GENETIC VARIATION
HIBRIDACION
HYBRIDATION
HYBRIDIZATION
Microscopy, Electron, Scanning
Molecular Sequence Data
MYCOSPHAERELLA GRAMINICOLA
Polymorphism, Genetic
PROGENY TESTING
PRUEBAS DE DESCENDENCIA
Random Amplified Polymorphic DNA Technique
Recombination, Genetic
REPRODUCCION SEXUAL
REPRODUCTION SEXUEE
SEXUAL REPRODUCTION
Spores, Fungal - ultrastructure
VARIACION GENETICA
VARIATION GENETIQUE
Xylariales - genetics
Xylariales - physiology
Xylariales - ultrastructure
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Summary:Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting from a single diploid nucleus were determined. A procedure to cross these isolates was developed to investigate the mating system. Three of six crosses were successful, and the segregation of mating types in accordance with the tetrad analysis strongly points to a bipolar heterothallic mating system in M. graminicola. Random ascospore progenies from the successful crosses, each comprising 54 isolates, were studied with three primers to determine the mode of inheritance of the RAPD markers. Mendelian segregation and recombination of RAPD markers was observed in all progenies.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050129