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Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola
Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting f...
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| Published in: | Current genetics 1996-08, Vol.30 (3), p.251-258 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c338t-4799d0792ee2e6c2916fc23555590f837d3e2bfb6471ff16d34b01ff807bdbb23 |
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| container_end_page | 258 |
| container_issue | 3 |
| container_start_page | 251 |
| container_title | Current genetics |
| container_volume | 30 |
| creator | Kema, G.H.J Verstappen, E.C.P Todorova, M Waalwijk, C. (DLO-Research Inst. for Plant Protection (IPO-DLO), Wageningen (Netherlands)) |
| description | Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting from a single diploid nucleus were determined. A procedure to cross these isolates was developed to investigate the mating system. Three of six crosses were successful, and the segregation of mating types in accordance with the tetrad analysis strongly points to a bipolar heterothallic mating system in M. graminicola. Random ascospore progenies from the successful crosses, each comprising 54 isolates, were studied with three primers to determine the mode of inheritance of the RAPD markers. Mendelian segregation and recombination of RAPD markers was observed in all progenies. |
| doi_str_mv | 10.1007/s002940050129 |
| format | article |
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(DLO-Research Inst. for Plant Protection (IPO-DLO), Wageningen (Netherlands))</creatorcontrib><title>Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>Monospore isolates of Mycosphaerella graminicola considered to originate from one ascus were analysed by the polymerase chain reaction (PCR) with 32 RAPD primers. Eighteen of these revealed three classes of polymorphisms, which enabled a RAPD-based tetrad analysis. Four pairs of isolates resulting from a single diploid nucleus were determined. A procedure to cross these isolates was developed to investigate the mating system. Three of six crosses were successful, and the segregation of mating types in accordance with the tetrad analysis strongly points to a bipolar heterothallic mating system in M. graminicola. Random ascospore progenies from the successful crosses, each comprising 54 isolates, were studied with three primers to determine the mode of inheritance of the RAPD markers. 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Three of six crosses were successful, and the segregation of mating types in accordance with the tetrad analysis strongly points to a bipolar heterothallic mating system in M. graminicola. Random ascospore progenies from the successful crosses, each comprising 54 isolates, were studied with three primers to determine the mode of inheritance of the RAPD markers. Mendelian segregation and recombination of RAPD markers was observed in all progenies.</abstract><cop>United States</cop><pmid>8753655</pmid><doi>10.1007/s002940050129</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1996-08, Vol.30 (3), p.251-258 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78228606 |
| source | Springer Nature - Connect here FIRST to enable access |
| subjects | Base Sequence Crosses, Genetic DNA Primers EPIDEMIOLOGIA EPIDEMIOLOGIE EPIDEMIOLOGY EPREUVE SUR LA DESCENDANCE Genetic Markers GENETIC VARIATION HIBRIDACION HYBRIDATION HYBRIDIZATION Microscopy, Electron, Scanning Molecular Sequence Data MYCOSPHAERELLA GRAMINICOLA Polymorphism, Genetic PROGENY TESTING PRUEBAS DE DESCENDENCIA Random Amplified Polymorphic DNA Technique Recombination, Genetic REPRODUCCION SEXUAL REPRODUCTION SEXUEE SEXUAL REPRODUCTION Spores, Fungal - ultrastructure VARIACION GENETICA VARIATION GENETIQUE Xylariales - genetics Xylariales - physiology Xylariales - ultrastructure |
| title | Successful crosses and molecular tetrad and progeny analyses demonstrate heterothallism in Mycosphaerella graminicola |
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