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Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT

Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine.We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleoti...

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Published in:	Journal of biochemistry (Tokyo) 1996-04, Vol.119 (4), p.811-816
Main Authors:	Matsugi, Jitsuhiro, Murao, Katsutoshi, Ishikura, Hisayuki
Format:	Article
Language:	English
Subjects:	Acylation Anticodon - chemistry Anticodon - genetics Bacillus subtilis Bacillus subtilis - genetics Base Sequence DNA, Bacterial - chemistry DNA, Bacterial - genetics FAB mass spectroscopy Genes, Bacterial - genetics Isoleucine - metabolism lysidine Lysine - analogs & derivatives Lysine - analysis Methionine - genetics Methionine - metabolism modified nucleotide Molecular Sequence Data Nucleic Acid Conformation Pyrimidine Nucleosides - analysis RNA, Transfer, Ile - genetics RNA, Transfer, Ile - isolation & purification RNA, Transfer, Ile - metabolism Sequence Analysis, RNA Transcription, Genetic tRNA
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container_issue	4
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container_title	Journal of biochemistry (Tokyo)
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creator	Matsugi, Jitsuhiro Murao, Katsutoshi Ishikura, Hisayuki
description	Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine.We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-β-D-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.
doi_str_mv	10.1093/oxfordjournals.jbchem.a021312
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Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-β-D-ribofuranosyl pyrimidine]. 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Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-β-D-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.</description><subject>Acylation</subject><subject>Anticodon - chemistry</subject><subject>Anticodon - genetics</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>Base Sequence</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>FAB mass spectroscopy</subject><subject>Genes, Bacterial - genetics</subject><subject>Isoleucine - metabolism</subject><subject>lysidine</subject><subject>Lysine - analogs & derivatives</subject><subject>Lysine - analysis</subject><subject>Methionine - genetics</subject><subject>Methionine - metabolism</subject><subject>modified nucleotide</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Pyrimidine Nucleosides - analysis</subject><subject>RNA, Transfer, Ile - genetics</subject><subject>RNA, Transfer, Ile - isolation & purification</subject><subject>RNA, Transfer, Ile - metabolism</subject><subject>Sequence Analysis, RNA</subject><subject>Transcription, Genetic</subject><subject>tRNA</subject><issn>0021-924X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkctuEzEUhr0AlVJ4BCRvYDfBl7l4FizSFEjVpBUQpIqN5bGPidOZcbE9qPD0dZSoEitWPvZ_8ZE-hN5SMqOk5e_9g_XB7PwURtXH2a7TWxhmijDKKXuGTkmeipaVty_Qyxh3-yvj_ASdiKbklahP0d1iq4LSCYL7q5LzI_YWK3w-w3HqkutdxGs3-oAvo-9h0m4EnL5ez_EFmEmDwTb4AaeL_LJUv934M4fXkLa5aW-dj8lpb3LtYr55hZ7bvCa8Pp5n6Punj5vFsljdfL5czFeFrphIRa10I4wFQ2mnBSsrUZpSdaIsK6tqSikD2-hKd5YT0gK0RmnKG6aE5UB4zc_Qu0PvffC_JohJDi5q6Hs1gp-ibATjomz-b6RVSyvSttn44WDUwccYwMr74AYV_khK5J6E_JeEPJCQRxI5_-b40dQNYJ7SRwxZLw66iwkenmQV7mTd8KaSy9sfcrk6_7b-srmSG_4IVVieYA</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Matsugi, Jitsuhiro</creator><creator>Murao, Katsutoshi</creator><creator>Ishikura, Hisayuki</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19960401</creationdate><title>Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT</title><author>Matsugi, Jitsuhiro ; Murao, Katsutoshi ; Ishikura, Hisayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-6ac78dfed11bc824584d4ab8445fa61112ef7c5cbf3009ee9dac1372a8f3e0363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acylation</topic><topic>Anticodon - chemistry</topic><topic>Anticodon - genetics</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>Base Sequence</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>FAB mass spectroscopy</topic><topic>Genes, Bacterial - genetics</topic><topic>Isoleucine - metabolism</topic><topic>lysidine</topic><topic>Lysine - analogs & derivatives</topic><topic>Lysine - analysis</topic><topic>Methionine - genetics</topic><topic>Methionine - metabolism</topic><topic>modified nucleotide</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Pyrimidine Nucleosides - analysis</topic><topic>RNA, Transfer, Ile - genetics</topic><topic>RNA, Transfer, Ile - isolation & purification</topic><topic>RNA, Transfer, Ile - metabolism</topic><topic>Sequence Analysis, RNA</topic><topic>Transcription, Genetic</topic><topic>tRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsugi, Jitsuhiro</creatorcontrib><creatorcontrib>Murao, Katsutoshi</creatorcontrib><creatorcontrib>Ishikura, Hisayuki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsugi, Jitsuhiro</au><au>Murao, Katsutoshi</au><au>Ishikura, Hisayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>119</volume><issue>4</issue><spage>811</spage><epage>816</epage><pages>811-816</pages><issn>0021-924X</issn><abstract>Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine.We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-β-D-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>8743586</pmid><doi>10.1093/oxfordjournals.jbchem.a021312</doi><tpages>6</tpages></addata></record>
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source	J-STAGE (Japan Science & Technology Information Aggregator, Electronic) - Open Access English articles; Oxford Journals Online
subjects	Acylation Anticodon - chemistry Anticodon - genetics Bacillus subtilis Bacillus subtilis - genetics Base Sequence DNA, Bacterial - chemistry DNA, Bacterial - genetics FAB mass spectroscopy Genes, Bacterial - genetics Isoleucine - metabolism lysidine Lysine - analogs & derivatives Lysine - analysis Methionine - genetics Methionine - metabolism modified nucleotide Molecular Sequence Data Nucleic Acid Conformation Pyrimidine Nucleosides - analysis RNA, Transfer, Ile - genetics RNA, Transfer, Ile - isolation & purification RNA, Transfer, Ile - metabolism Sequence Analysis, RNA Transcription, Genetic tRNA
title	Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT
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