Loading…
A one-step, separation-free amperometric enzyme immunosensor
A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoa...
Saved in:
| Published in: | Biosensors & bioelectronics 1996, Vol.11 (4), p.409-417 |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493 |
| container_end_page | 417 |
| container_issue | 4 |
| container_start_page | 409 |
| container_title | Biosensors & bioelectronics |
| container_volume | 11 |
| creator | Ivnitski, D. Rishpon, J. |
| description | A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I
2/I
−); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically
in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5–20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a ‘sandwich’ immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml
−1. |
| doi_str_mv | 10.1016/0956-5663(96)82736-9 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78243829</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0956566396827369</els_id><sourcerecordid>78243829</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMoun78A4UeRBSsTj6aNCCCiF8geNFzyKYTiGybmnQF_fV23WWPeprD-8w7w0PIIYULClRegq5kWUnJT7U8q5nistQbZEJrxUvBeLVJJmtkh-zm_A4AimrYJtu1EnIEJ-TqpogdlnnA_rzI2NtkhxC70ifEwrY9ptjikIIrsPv-arEIbTvvYsYux7RPtrydZTxYzT3ydn_3evtYPr88PN3ePJdOVHwoGXjnAGpArzlnmjvd6KnQHllTMW-5qt0UqfISalspUJxqxm1jneC8EZrvkZNlb5_ixxzzYNqQHc5mtsM4z0bVTPCa_Q_Ssb3iACMolqBLMeeE3vQptDZ9GQpmYdcs1JmFOqOl-bVrFv1Hq_75tMVmvbTSOebHq9xmZ2c-2c6FvMbGyyBBjNj1EsNR2mfAZLIL2DlsQkI3mCaGv__4AV1ilQg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15705300</pqid></control><display><type>article</type><title>A one-step, separation-free amperometric enzyme immunosensor</title><source>ScienceDirect Journals</source><creator>Ivnitski, D. ; Rishpon, J.</creator><creatorcontrib>Ivnitski, D. ; Rishpon, J.</creatorcontrib><description>A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I
2/I
−); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically
in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5–20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a ‘sandwich’ immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml
−1.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/0956-5663(96)82736-9</identifier><identifier>PMID: 8746187</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>amperometry ; Antibodies ; Biological and medical sciences ; Biosensing Techniques ; Biosensors ; Biotechnology ; Cost Control ; disposable electrode ; Electrochemistry ; enzyme immunoassay ; Fundamental and applied biological sciences. Psychology ; Glucose Oxidase ; Immunoenzyme Techniques - economics ; immunosensor ; Methods. Procedures. Technologies ; Sensitivity and Specificity ; signal-amplification systems ; Time Factors ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 1996, Vol.11 (4), p.409-417</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493</citedby><cites>FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3000604$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8746187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivnitski, D.</creatorcontrib><creatorcontrib>Rishpon, J.</creatorcontrib><title>A one-step, separation-free amperometric enzyme immunosensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I
2/I
−); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically
in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5–20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a ‘sandwich’ immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml
−1.</description><subject>amperometry</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cost Control</subject><subject>disposable electrode</subject><subject>Electrochemistry</subject><subject>enzyme immunoassay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose Oxidase</subject><subject>Immunoenzyme Techniques - economics</subject><subject>immunosensor</subject><subject>Methods. Procedures. Technologies</subject><subject>Sensitivity and Specificity</subject><subject>signal-amplification systems</subject><subject>Time Factors</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMoun78A4UeRBSsTj6aNCCCiF8geNFzyKYTiGybmnQF_fV23WWPeprD-8w7w0PIIYULClRegq5kWUnJT7U8q5nistQbZEJrxUvBeLVJJmtkh-zm_A4AimrYJtu1EnIEJ-TqpogdlnnA_rzI2NtkhxC70ifEwrY9ptjikIIrsPv-arEIbTvvYsYux7RPtrydZTxYzT3ydn_3evtYPr88PN3ePJdOVHwoGXjnAGpArzlnmjvd6KnQHllTMW-5qt0UqfISalspUJxqxm1jneC8EZrvkZNlb5_ixxzzYNqQHc5mtsM4z0bVTPCa_Q_Ssb3iACMolqBLMeeE3vQptDZ9GQpmYdcs1JmFOqOl-bVrFv1Hq_75tMVmvbTSOebHq9xmZ2c-2c6FvMbGyyBBjNj1EsNR2mfAZLIL2DlsQkI3mCaGv__4AV1ilQg</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Ivnitski, D.</creator><creator>Rishpon, J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>A one-step, separation-free amperometric enzyme immunosensor</title><author>Ivnitski, D. ; Rishpon, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>amperometry</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cost Control</topic><topic>disposable electrode</topic><topic>Electrochemistry</topic><topic>enzyme immunoassay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose Oxidase</topic><topic>Immunoenzyme Techniques - economics</topic><topic>immunosensor</topic><topic>Methods. Procedures. Technologies</topic><topic>Sensitivity and Specificity</topic><topic>signal-amplification systems</topic><topic>Time Factors</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ivnitski, D.</creatorcontrib><creatorcontrib>Rishpon, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivnitski, D.</au><au>Rishpon, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A one-step, separation-free amperometric enzyme immunosensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1996</date><risdate>1996</risdate><volume>11</volume><issue>4</issue><spage>409</spage><epage>417</epage><pages>409-417</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A new one-step, separation-free, amperometric enzyme immunosensor is described. The sensor consists of an antibody electrode that is low cost, disposable, and operates without washing or separation steps. The immunosensor combines the following signal-amplification systems: enzyme-channeling immunoassay; accumulation of the redox mediators (I
2/I
−); cyclic regeneration of an enzyme (peroxidase) substrate at the (polyethylenimine) polymer/electrode interface; and control of the hydrodynamic conditions at the interface of the antibody electrode. The immunological reactions were monitored electrochemically
in situ, and the binding curves were directly visualized on a computer screen. The complete immunoassay can be performed in 5–20 min depending on the complexity of the immunological reactions. Model systems using rabbit IgG and human luteinizing hormone (hLH) in a ‘sandwich’ immunoassay revealed that the immunosensor can detect concentrations of hLH in human serum as low as 1 ng ml
−1.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>8746187</pmid><doi>10.1016/0956-5663(96)82736-9</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 1996, Vol.11 (4), p.409-417 |
| issn | 0956-5663 1873-4235 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78243829 |
| source | ScienceDirect Journals |
| subjects | amperometry Antibodies Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Cost Control disposable electrode Electrochemistry enzyme immunoassay Fundamental and applied biological sciences. Psychology Glucose Oxidase Immunoenzyme Techniques - economics immunosensor Methods. Procedures. Technologies Sensitivity and Specificity signal-amplification systems Time Factors Various methods and equipments |
| title | A one-step, separation-free amperometric enzyme immunosensor |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T12%3A15%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20one-step,%20separation-free%20amperometric%20enzyme%20immunosensor&rft.jtitle=Biosensors%20&%20bioelectronics&rft.au=Ivnitski,%20D.&rft.date=1996&rft.volume=11&rft.issue=4&rft.spage=409&rft.epage=417&rft.pages=409-417&rft.issn=0956-5663&rft.eissn=1873-4235&rft_id=info:doi/10.1016/0956-5663(96)82736-9&rft_dat=%3Cproquest_cross%3E78243829%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c453t-20fcc0080ef933293c9d9b49fe2d52fa378cbe17f608a570731923adac433d493%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15705300&rft_id=info:pmid/8746187&rfr_iscdi=true |