Hypoxia precedes the development of experimental preretinal neovascularization

Although the mechanism of preretinal neovascular growth in the cell-injected rabbit eye model is not known, it has been proposed that the initial vasodilation and eventual development of neovascularization may be attributable to inflammatory mediators. However, an alternative explanation involving h...

Full description

Saved in:
Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 1996, Vol.234 (1), p.43-46
Main Authors: HANDA, J. T, BERKOWITZ, B. A, WILSON, C. A, ANDO, NOBURO, SEN, H. A, JAFFE, G. J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Disease Models, Animal
Female
Fibroblasts - pathology
Fluorocarbons
Hypoxia - etiology
Hypoxia - metabolism
Magnetic Resonance Spectroscopy
Male
Medical sciences
Ophthalmology
Oxygen - metabolism
Rabbits
Retinal Neovascularization - etiology
Retinal Neovascularization - metabolism
Retinopathies
Skin - cytology
Vitreous Body - metabolism
Vitreous Body - pathology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although the mechanism of preretinal neovascular growth in the cell-injected rabbit eye model is not known, it has been proposed that the initial vasodilation and eventual development of neovascularization may be attributable to inflammatory mediators. However, an alternative explanation involving hypoxia has not been considered. The purpose of this study was to measure preretinal oxygen tension prior to the development of preretinal neovascularization in the cell-injected rabbit eye. In the rabbit, intravitreous injections of 250,000 homologous dermal fibroblasts were performed on one eye; the fellow (control) eye was injected with vehicle. Preretinal oxygen tension over the myelin wing was measured using 19F-NMR spectroscopy of a 30-microliters droplet of perfluorocarbon previously injected into the preretinal vitreous. Compared to control eyes, fibroblast-injected eyes showed a 1.7-fold decrease in preretinal oxygen tension from the first time studied (1 day after cell injection) through the development of visible neovascularization. Hypoxia occurred without coexisting ophthalmoscopic evidence of vascular occlusion or, on days 1 and 3 after cell injection, retinal detachment. This result demonstrates for the first time that preretinal hypoxia precedes the development of preretinal neovascularization in the fibroblast-injected rabbit eye.
ISSN:0721-832X
1435-702X
DOI:10.1007/BF00186517