Volume of blood required to detect common neonatal pathogens

OBJECTIVE: To determine the minimum volume of blood and the absolute number of organisms required for detection of bacteremia and fungemia by an automated colorimetric blood culture system (BacT/Alert, Organon Teknika). DESIGN: Common neonatal pathogens, Escherichia coli, Streptococcus agalactiae (g...

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Bibliographic Details
Published in:The Journal of pediatrics 1996-08, Vol.129 (2), p.275-278
Main Authors: Schelonka, Robert L., Chai, Michele K., Yoder, Bradley A., Hensley, Donna, Brockett, Royce M., Ascher, David P.
Format: Article
Language:English
Subjects:
Bacteremia - blood
Bacteremia - microbiology
Biological and medical sciences
Candida albicans - isolation & purification
Candidiasis - blood
Colony Count, Microbial
Colorimetry
Culture Media
Escherichia coli - isolation & purification
Escherichia coli Infections - blood
False Positive Reactions
Fungemia - blood
Fungemia - microbiology
Humans
Infant, Newborn - blood
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Poisson Distribution
Probability
Risk Factors
Sepsis - microbiology
Staphylococcal Infections - blood
Staphylococcus epidermidis - isolation & purification
Streptococcal Infections - blood
Streptococcus agalactiae - isolation & purification
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Summary:OBJECTIVE: To determine the minimum volume of blood and the absolute number of organisms required for detection of bacteremia and fungemia by an automated colorimetric blood culture system (BacT/Alert, Organon Teknika). DESIGN: Common neonatal pathogens, Escherichia coli, Streptococcus agalactiae (group B streptococcus [GBS]: one American Type Culture Collection [ATCC] strain and one clinical isolate), Staphylococcus epidermidis, and Candida albicans, were seeded into blood to produce bacteremia or fungemia with low colony counts (1 to 3 colony-forming units [CFU] per milliliter) and ultra-low colony counts (
ISSN:0022-3476
1097-6833
DOI:10.1016/S0022-3476(96)70254-8