Glucocorticoid upregulation of glutamate dehydrogenase gene expression in vitro in astrocytes

Glutamate, the major excitatory neurotransmitter, is preferentially catabolized in astrocytes by glutamate dehydrogenase (GDH). Treatment of an astrocytic cell line with hydrocortisone (10 −5 M) resulted in increased expression of GDH mRNA. Transfection of the cells with truncated parts of the GDH p...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1996-04, Vol.37 (1), p.324-328
Main Authors: Hardin-Pouzet, H., Giraudon, P., Belin, M.F., Didier-Bazes, M.
Format: Article
Language:English
Subjects:
Animals
Astrocyte
Astrocytes - drug effects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
GDH promoter
Gene Expression - drug effects
Glucocorticoid
Glucocorticoids - pharmacology
Glutamate Dehydrogenase - drug effects
Glutamate metabolis
Hydrocortisone - pharmacology
In Vitro Techniques
Isolated neuron and nerve. Neuroglia
Mice
Mice, Inbred Strains
Neuron-glia interactions
Up-Regulation
Vertebrates: nervous system and sense organs
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Summary:Glutamate, the major excitatory neurotransmitter, is preferentially catabolized in astrocytes by glutamate dehydrogenase (GDH). Treatment of an astrocytic cell line with hydrocortisone (10 −5 M) resulted in increased expression of GDH mRNA. Transfection of the cells with truncated parts of the GDH promoter showed that genomic responsive elements activated by hydrocortisone are localized in the −557/+1 region of the promoter. This control of GDH expression by glucocorticoids may be involved in their protective effect against glutamate excitotoxicity.
ISSN:0169-328X
1872-6941
DOI:10.1016/0169-328X(95)00327-O