Elevated DNase I Levels in Human Idiopathic Dilated Cardiomyopathy: an Indicator of Apoptosis ?

This is the first report to determine deoxyribonuclease I (DNase I) levels in the human myocardium and the first to demonstrate an increased DNase I level associated with end-stage heart failure due to idiopathic dilated cardiomyopathy (IDCM) compared to non-diseased heart samples. Left ventricular...

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Bibliographic Details
Published in:Journal of molecular and cellular cardiology 1996-01, Vol.28 (1), p.95-101
Main Authors: Yao, Mu, Keogh, Anne, Spratt, Phillip, dos Remedios, Cristobal G., Kie ßling, Peter C.
Format: Article
Language:English
Subjects:
Actins - pharmacology
Adult
Apoptosis
Biomarkers
Cardiomyopathy, Dilated - enzymology
Cardiomyopathy, Dilated - pathology
Deoxyribonuclease I
Deoxyribonuclease I - analysis
Deoxyribonuclease I - metabolism
Deoxyribonucleic acid
Dilated cardiomyopathy
Female
Heart Ventricles
Humans
Kinetics
Male
Middle Aged
Myocardium - enzymology
Myocardium - pathology
Reference Values
Sex Characteristics
Zymogram
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Summary:This is the first report to determine deoxyribonuclease I (DNase I) levels in the human myocardium and the first to demonstrate an increased DNase I level associated with end-stage heart failure due to idiopathic dilated cardiomyopathy (IDCM) compared to non-diseased heart samples. Left ventricular samples were obtained following transplantation from failing hearts of 13 patients diagnosed with IDCM and from four unused donor hearts. Using a zymogram technique, we show that the DNase I levels of the IDCM heart samples were significantly elevated (range 0.65 –2.75 pg DNase I / μg protein, mean ± s.e.of 1.69 ±0.22 pg / μg) compared to four non-diseased, donor heart samples (range 0.12 –0.35 pg / μg protein, mean ± s.e.of 0.22 ±0.05 pg / μg). The DNase I extracted from heart tissue was characterized by: (1) a co-migration with bovine pancreatic DNase I; (2) a pH dependence consistent with DNase I; (3) a dependence of its activity on both Ca 2 +and Mg 2 +and an inhibition by Zn 2 +; and (4) an inhibition of its activity in the presence of monomeric rabbit skeletal muscle actin. The elevated DNase I levels associated with heart failure due to IDCM suggests that apoptosis may be implicated in pathophysiology of this disorder.
ISSN:0022-2828
1095-8584
DOI:10.1006/jmcc.1996.0009