A Common PDGF Receptor is Activated by Homodimeric A and B Forms of PDGF

The human platelet-derived growth factor (PDGF) receptor complementary DNA was cloned and expressed by transfection of Chinese hamster ovary (CHO) fibroblasts. The ability of CHO cells expressing the human receptor complementary DNA (CHO-HR5) to interact with different recombinant forms of PDGF (AA...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-06, Vol.240 (4858), p.1532-1534
Main Authors: Escobedo, Jaime A., Navankasatussas, Sutip, Cousens, Lawrence S., Coughlin, Shaun R., Bell, Graeme I., Williams, Lewis T.
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Antibodies
BALB 3T3 cells
Biochemistry
Biological and medical sciences
Blood
Cell Division - drug effects
Cell Line
Cell membranes
Cell receptors
Cell structures and functions
Cells, Cultured
Chloroplasts
CHO cells
Complementary DNA
DNA
DNA Replication - drug effects
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Macromolecular Substances
Membrane proteins
Mice
Molecular and cellular biology
Phosphorylation
Platelet-derived growth factor
Platelet-Derived Growth Factor - genetics
Platelet-Derived Growth Factor - metabolism
Platelet-Derived Growth Factor - pharmacology
Protein research
Protein-Tyrosine Kinases - metabolism
Receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Receptors, Platelet-Derived Growth Factor
Recombinant Proteins - pharmacology
Transfection
Yeasts
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Summary:The human platelet-derived growth factor (PDGF) receptor complementary DNA was cloned and expressed by transfection of Chinese hamster ovary (CHO) fibroblasts. The ability of CHO cells expressing the human receptor complementary DNA (CHO-HR5) to interact with different recombinant forms of PDGF (AA and BB homodimers) was tested. Both forms of PDGF bind to the transfected receptor, stimulate the receptor tyrosine kinase activity, and elicit a mitogenic response in a manner that was indistinguishable from the responses of Balb/c 3T3 cells. The data indicate that the tyrosine kinase and mitogenic responses of the Balb/c 3T3 cells to AA and BB forms of PDGF can be attributed to a single type of receptor and show that the AA form, like the BB form, is a true mitogen.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2836953