Purification of acid phosphatase I from germinating seeds of Vigna sinensis

Acid phosphatase I (AP-I) is the major isoform of Vigna acid phosphatase. It is constitutively expressed in seed cotyledons during germination. AP-I was separated from other isoforms and purified to homogeneity by three simple purification steps; (NH 4) 2SO 4 precipitation, and phosphocellulose and...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1996-04, Vol.41 (6), p.1457-1458
Main Authors: Biswas, Tapan K., Promo, Michele, Biswas, Bani
Format: Article
Language:English
Subjects:
Acid Phosphatase - isolation & purification
Acid Phosphatase - metabolism
Ammonium Sulfate
Biological and medical sciences
Biotechnology
Cations - pharmacology
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Enzymes
Fabaceae - enzymology
Fundamental and applied biological sciences. Psychology
Isoenzymes - isolation & purification
Isoenzymes - metabolism
Kinetics
Metabolism
Plant physiology and development
Plants, Medicinal
Seeds
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Description
Summary:Acid phosphatase I (AP-I) is the major isoform of Vigna acid phosphatase. It is constitutively expressed in seed cotyledons during germination. AP-I was separated from other isoforms and purified to homogeneity by three simple purification steps; (NH 4) 2SO 4 precipitation, and phosphocellulose and DEAE-cellulose column chromatography. The activity of AP-I was not affected by 1 mM Mg 2+, Mn 2+, Ca 2+, Co 2+ or Pb 2+, but severely inhibited by 1 mM Cu 2+, Fe 3+, Hg 2+, Mo 6+ or Zn 2+. AP-I has both phosphatase and pyrophosphatase activities, and is highly stable even at 50°.
ISSN:0031-9422
1873-3700
DOI:10.1016/0031-9422(95)00826-8