An Alternative Method for the Culture of Diplostomum spathaceum (Trematoda) in Chick Embryos

Metacercariae of Diplostomum spathaceum were maintained in the allantoic cavities of chick embryos for 15 days. Some embryos had 0.2 ml chicken serum added to the allantoic cavity each day. Although the level of development varied considerably, worms from embryos with added serum developed hind bodi...

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Bibliographic Details
Published in:The Journal of parasitology 1988-06, Vol.74 (3), p.504-505
Main Authors: Irwin, S. W. B., Saville, D. H.
Format: Article
Language:English
Subjects:
Allantois - parasitology
Animals
Blood
Chick Embryo
Chicks
Chorioallantoic membrane
Culture Techniques - methods
Eggs
Embryos
Metacercariae
Parasites
Parasitology
Research Notes
Time Factors
Trematoda - anatomy & histology
Trematoda - growth & development
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Summary:Metacercariae of Diplostomum spathaceum were maintained in the allantoic cavities of chick embryos for 15 days. Some embryos had 0.2 ml chicken serum added to the allantoic cavity each day. Although the level of development varied considerably, worms from embryos with added serum developed hind bodies that were substantially larger than those of parasites maintained without added serum. There was no evidence that any worm ingested blood, and only 1 individual, from the serum-augmented group, became ovigerous.
ISSN:0022-3395
1937-2345
DOI:10.2307/3282067