Expression of integrins in marmoset ( Callithrix jacchus) ovary during folliculogenesis

Integrins were localized immunohistochemically in marmoset ovaries ( Callithrix jacchus) of defined cycle stages. With monoclonal antibodies against β1, α2, α3, and α6 integrin subunits, immunoreactivity was predominantly found in ovaries of the follicular phase. In the luteal phase, non-luteal cell...

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Bibliographic Details
Published in:Tissue & cell 1996-08, Vol.28 (4), p.379-385
Main Authors: Giebel, J., de Souza, P., Rune, G.M.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
atresia
Callithrix - anatomy & histology
Callithrix - physiology
Estrus - physiology
Female
Follicular Phase - physiology
immunocytochemistry
Immunohistochemistry
integrins
Integrins - analysis
Integrins - biosynthesis
Integrins - immunology
Luteal Phase - physiology
luteinization
Male
Marmoset monkey
Ovary - chemistry
Pregnancy
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Summary:Integrins were localized immunohistochemically in marmoset ovaries ( Callithrix jacchus) of defined cycle stages. With monoclonal antibodies against β1, α2, α3, and α6 integrin subunits, immunoreactivity was predominantly found in ovaries of the follicular phase. In the luteal phase, non-luteal cells, e.g. fibroblasts or endothelial cells expressed β1, α2, or α6 integrins. Immunostaining for the β1 subunit was strongest in granulosa cells of all growing follicles. Weak immunoreactivity was found in granulosa cells of atretic follicles, theca and interstitial cells. With the α2 antibody, binding was evident in granulosa cells of many, but not of all, primordial and primary follicles. Expression of α2 was also found in luteinized granulosa cells of tertiary follicles. In growing follicles, immunoreactivity of α3 was restricted to granulosa cells of early secondary follicles. Within the epithelium, staining was almost exclusively found in granulosa cells bordering on the basal granulosa cell layer. In advanced stages of atresia, granulosa cells of antral follicles expressed the α3 subunit. Integrin α6 expression was evident in granulosa cells of all growing follicles but was absent during advanced stages of degeneration. The specific expression patterns of integrins suggest that these matrix receptors play a regulatory role during cyclic events in primate ovaries.
ISSN:0040-8166
1532-3072
DOI:10.1016/S0040-8166(96)80024-3