Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes

• Published in: FEBS letters 1988-05, Vol.232 (2), p.347-350
• Main Authors: Castell, José V., Gómez-Lechón, Maria J., David, Martina, Hirano, Toshio, Kishimoto, Tadamitsu, Heinrich, Peter C.
• Format: Article
• Language: English
• Subjects: Acute-phase protein; Acute-Phase Proteins - biosynthesis; Adult; alpha 1-Antichymotrypsin - biosynthesis; alpha 1-Antitrypsin - biosynthesis; alpha-Macroglobulins - biosynthesis; B-cell stimulatory factor 2; Biological and medical sciences; BSF-2, B-cell stimulatory factor 2; C-Reactive Protein - biosynthesis; Cells, Cultured; Dexamethasone - pharmacology; Electrophoresis, Polyacrylamide Gel; Fibrinogen - biosynthesis; Fundamental and applied biological sciences. Psychology; Haptoglobins - biosynthesis; Hepatocyte stimulating factor; HSF, hepatocyte-stimulating factor; Human hepatocyte; Humans; IL-6, interleukin-6; Immunosorbent Techniques; Inflammation; Interleukin-6; Interleukins - pharmacology; Liver - metabolism; Methionine - metabolism; Molecular and cellular biology; Orosomucoid - biosynthesis; Recombinant Proteins - pharmacology; rhIL-6, recombinant human interleukin-6; Serum Amyloid A Protein - biosynthesis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(88)80766-X

Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [ 35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α 1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α 1-antitrypsin (2.7-fold) and α 1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α 1-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α 1-antichymotrypsin, α 1-antitrypsin and α 1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.