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Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes
Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [ 35S]methionine acute phase protein synthesis wa...
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| Published in: | FEBS letters 1988-05, Vol.232 (2), p.347-350 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c643X-a90d5054fcacdf6fb9f99e7b6edeff8aaa023461a81529ed703170146f983ae13 |
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| cites | cdi_FETCH-LOGICAL-c643X-a90d5054fcacdf6fb9f99e7b6edeff8aaa023461a81529ed703170146f983ae13 |
| container_end_page | 350 |
| container_issue | 2 |
| container_start_page | 347 |
| container_title | FEBS letters |
| container_volume | 232 |
| creator | Castell, José V. Gómez-Lechón, Maria J. David, Martina Hirano, Toshio Kishimoto, Tadamitsu Heinrich, Peter C. |
| description | Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [
35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α
1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α
1-antitrypsin (2.7-fold) and α
1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α
1-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α
1-antichymotrypsin, α
1-antitrypsin and α
1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells. |
| doi_str_mv | 10.1016/0014-5793(88)80766-X |
| format | article |
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35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α
1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α
1-antitrypsin (2.7-fold) and α
1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α
1-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α
1-antichymotrypsin, α
1-antitrypsin and α
1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(88)80766-X</identifier><identifier>PMID: 2454206</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acute-phase protein ; Acute-Phase Proteins - biosynthesis ; Adult ; alpha 1-Antichymotrypsin - biosynthesis ; alpha 1-Antitrypsin - biosynthesis ; alpha-Macroglobulins - biosynthesis ; B-cell stimulatory factor 2 ; Biological and medical sciences ; BSF-2, B-cell stimulatory factor 2 ; C-Reactive Protein - biosynthesis ; Cells, Cultured ; Dexamethasone - pharmacology ; Electrophoresis, Polyacrylamide Gel ; Fibrinogen - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Haptoglobins - biosynthesis ; Hepatocyte stimulating factor ; HSF, hepatocyte-stimulating factor ; Human hepatocyte ; Humans ; IL-6, interleukin-6 ; Immunosorbent Techniques ; Inflammation ; Interleukin-6 ; Interleukins - pharmacology ; Liver - metabolism ; Methionine - metabolism ; Molecular and cellular biology ; Orosomucoid - biosynthesis ; Recombinant Proteins - pharmacology ; rhIL-6, recombinant human interleukin-6 ; Serum Amyloid A Protein - biosynthesis</subject><ispartof>FEBS letters, 1988-05, Vol.232 (2), p.347-350</ispartof><rights>1988</rights><rights>FEBS Letters 232 (1988) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c643X-a90d5054fcacdf6fb9f99e7b6edeff8aaa023461a81529ed703170146f983ae13</citedby><cites>FETCH-LOGICAL-c643X-a90d5054fcacdf6fb9f99e7b6edeff8aaa023461a81529ed703170146f983ae13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457938880766X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3535,27903,27904,45759</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7101316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2454206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castell, José V.</creatorcontrib><creatorcontrib>Gómez-Lechón, Maria J.</creatorcontrib><creatorcontrib>David, Martina</creatorcontrib><creatorcontrib>Hirano, Toshio</creatorcontrib><creatorcontrib>Kishimoto, Tadamitsu</creatorcontrib><creatorcontrib>Heinrich, Peter C.</creatorcontrib><title>Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [
35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α
1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α
1-antitrypsin (2.7-fold) and α
1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α
1-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α
1-antichymotrypsin, α
1-antitrypsin and α
1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.</description><subject>Acute-phase protein</subject><subject>Acute-Phase Proteins - biosynthesis</subject><subject>Adult</subject><subject>alpha 1-Antichymotrypsin - biosynthesis</subject><subject>alpha 1-Antitrypsin - biosynthesis</subject><subject>alpha-Macroglobulins - biosynthesis</subject><subject>B-cell stimulatory factor 2</subject><subject>Biological and medical sciences</subject><subject>BSF-2, B-cell stimulatory factor 2</subject><subject>C-Reactive Protein - biosynthesis</subject><subject>Cells, Cultured</subject><subject>Dexamethasone - pharmacology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fibrinogen - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haptoglobins - biosynthesis</subject><subject>Hepatocyte stimulating factor</subject><subject>HSF, hepatocyte-stimulating factor</subject><subject>Human hepatocyte</subject><subject>Humans</subject><subject>IL-6, interleukin-6</subject><subject>Immunosorbent Techniques</subject><subject>Inflammation</subject><subject>Interleukin-6</subject><subject>Interleukins - pharmacology</subject><subject>Liver - metabolism</subject><subject>Methionine - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Orosomucoid - biosynthesis</subject><subject>Recombinant Proteins - pharmacology</subject><subject>rhIL-6, recombinant human interleukin-6</subject><subject>Serum Amyloid A Protein - biosynthesis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhi0EKkvLPwDJB4TaQ7p2PhzngkSrhlZaCYkWaW_WrDNmDYmztRPQ_nucbrRH6MUjz7zvY88MIe84u-SMiyVjPE-KssrOpbyQrBQiWb8gCy7LLMlyIV-SxVHymrwJ4SeLd8mrE3KS5kWeMrEg4zfUfbexDtxAt2MHjlo3oG9x_GVdIuj53SoRy6v7OkmXt_f1BfX4Y2xhwECHLdKwdzEEG2hvKOhxQLrbQoin7we0LkTczN3iDoZe76P1jLwy0AZ8O8dT8r2-ebi-TVZfv9xdf14lWuTZOoGKNQUrcqNBN0aYTWWqCsuNwAaNkQDA0tgpB8mLtMKmZBkvY8vCVDID5Nkp-Xjgxt88jhgG1dmgsW3BYT8GVcpURH_-XyEvOGcpm4j5Qah9H4JHo3beduD3ijM1rUVNM1fTzJWU6mktah1t72f-uOmwOZrmPcT6h7kOQUNrPDhtw1FWRnLGJ1l9kP2xLe6f9bSqb67SqTDlpXzKTv_5dABhnP5vi14FbdFpbKxHPaimt_9u6C_0LryQ</recordid><startdate>19880523</startdate><enddate>19880523</enddate><creator>Castell, José V.</creator><creator>Gómez-Lechón, Maria J.</creator><creator>David, Martina</creator><creator>Hirano, Toshio</creator><creator>Kishimoto, Tadamitsu</creator><creator>Heinrich, Peter C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19880523</creationdate><title>Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes</title><author>Castell, José V. ; Gómez-Lechón, Maria J. ; David, Martina ; Hirano, Toshio ; Kishimoto, Tadamitsu ; Heinrich, Peter C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c643X-a90d5054fcacdf6fb9f99e7b6edeff8aaa023461a81529ed703170146f983ae13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acute-phase protein</topic><topic>Acute-Phase Proteins - biosynthesis</topic><topic>Adult</topic><topic>alpha 1-Antichymotrypsin - biosynthesis</topic><topic>alpha 1-Antitrypsin - biosynthesis</topic><topic>alpha-Macroglobulins - biosynthesis</topic><topic>B-cell stimulatory factor 2</topic><topic>Biological and medical sciences</topic><topic>BSF-2, B-cell stimulatory factor 2</topic><topic>C-Reactive Protein - biosynthesis</topic><topic>Cells, Cultured</topic><topic>Dexamethasone - pharmacology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fibrinogen - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haptoglobins - biosynthesis</topic><topic>Hepatocyte stimulating factor</topic><topic>HSF, hepatocyte-stimulating factor</topic><topic>Human hepatocyte</topic><topic>Humans</topic><topic>IL-6, interleukin-6</topic><topic>Immunosorbent Techniques</topic><topic>Inflammation</topic><topic>Interleukin-6</topic><topic>Interleukins - pharmacology</topic><topic>Liver - metabolism</topic><topic>Methionine - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Orosomucoid - biosynthesis</topic><topic>Recombinant Proteins - pharmacology</topic><topic>rhIL-6, recombinant human interleukin-6</topic><topic>Serum Amyloid A Protein - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castell, José V.</creatorcontrib><creatorcontrib>Gómez-Lechón, Maria J.</creatorcontrib><creatorcontrib>David, Martina</creatorcontrib><creatorcontrib>Hirano, Toshio</creatorcontrib><creatorcontrib>Kishimoto, Tadamitsu</creatorcontrib><creatorcontrib>Heinrich, Peter C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castell, José V.</au><au>Gómez-Lechón, Maria J.</au><au>David, Martina</au><au>Hirano, Toshio</au><au>Kishimoto, Tadamitsu</au><au>Heinrich, Peter C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1988-05-23</date><risdate>1988</risdate><volume>232</volume><issue>2</issue><spage>347</spage><epage>350</epage><pages>347-350</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [
35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, α
1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for α
1-antitrypsin (2.7-fold) and α
1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on α
1-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, α
1-antichymotrypsin, α
1-antitrypsin and α
1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2454206</pmid><doi>10.1016/0014-5793(88)80766-X</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1988-05, Vol.232 (2), p.347-350 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Acute-phase protein Acute-Phase Proteins - biosynthesis Adult alpha 1-Antichymotrypsin - biosynthesis alpha 1-Antitrypsin - biosynthesis alpha-Macroglobulins - biosynthesis B-cell stimulatory factor 2 Biological and medical sciences BSF-2, B-cell stimulatory factor 2 C-Reactive Protein - biosynthesis Cells, Cultured Dexamethasone - pharmacology Electrophoresis, Polyacrylamide Gel Fibrinogen - biosynthesis Fundamental and applied biological sciences. Psychology Haptoglobins - biosynthesis Hepatocyte stimulating factor HSF, hepatocyte-stimulating factor Human hepatocyte Humans IL-6, interleukin-6 Immunosorbent Techniques Inflammation Interleukin-6 Interleukins - pharmacology Liver - metabolism Methionine - metabolism Molecular and cellular biology Orosomucoid - biosynthesis Recombinant Proteins - pharmacology rhIL-6, recombinant human interleukin-6 Serum Amyloid A Protein - biosynthesis |
| title | Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes |
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