Molecular Cloning and Characterization of the Human Anaphylatoxin C3a Receptor

In a human neutrophil cDNA library, an orphan G-protein-coupled receptor, HNFAG09, with 37% nucleotide identity to the C5a receptor (C5a-R, CD88) was identified. A novel feature of this gene, unlike C5a-R and other G-protein-coupled receptors, is the presence of an extraordinarily large predicted ex...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-08, Vol.271 (34), p.20231-20234
Main Authors: Ames, Robert S., Li, Yi, Sarau, Henry M., Nuthulaganti, Paru, Foley, James J., Ellis, Catherine, Zeng, Zhizhen, Su, Kui, Jurewicz, Anthony J., Hertzberg, Robert P., Bergsma, Derk J., Kumar, Chandrika
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, CD - genetics
Base Sequence
Cloning, Molecular
Complement C3a - metabolism
DNA Primers - chemistry
Gene Expression
Humans
Membrane Glycoproteins - genetics
Membrane Proteins
Molecular Sequence Data
Peptides - chemistry
Rats
Receptor, Anaphylatoxin C5a
Receptors, Complement - genetics
Recombinant Proteins
RNA, Messenger - genetics
Transfection
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Summary:In a human neutrophil cDNA library, an orphan G-protein-coupled receptor, HNFAG09, with 37% nucleotide identity to the C5a receptor (C5a-R, CD88) was identified. A novel feature of this gene, unlike C5a-R and other G-protein-coupled receptors, is the presence of an extraordinarily large predicted extracellular loop comprised of in excess of 160 amino acid residues between transmembrane domains 4 and 5. Northern blot analysis revealed that expression of mRNA for this receptor in human tissues, while similar, was distinct from C5a-R expression. Although there were differences in expression, transcripts for both receptors were detected in tissues throughout the body and the central nervous system. Mammalian cells stably expressing HNFAG09 specifically bound 125I-C3a and responded to a C3a carboxyl-terminal analogue synthetic peptide and to human C3a but not to rC5a with a robust calcium mobilization response. HNFAG09 encodes the human anaphylatoxin C3a receptor.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.34.20231