Sequence of centromere separation: differential replication of pericentric heterochromatin in multicentric chromosomes

The dicentric and multicentric chromosomes in L cells and a brain tumor cell line of mouse display only one site of kinetochore formation associated with the 'active' centromere. The accessory or 'inactive' centromeres show premature separation. These cell lines were treated with...

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Bibliographic Details
Published in:Chromosoma 1988-01, Vol.96 (4), p.311-317
Main Authors: VIG, B. K, BROCCOLI, D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Neoplasms
Bromodeoxyuridine
Cell Line
Centromere - ultrastructure
Chromatin. Chromosome
Chromosomes - ultrastructure
DNA Replication
Fundamental and applied biological sciences. Psychology
Heterochromatin - ultrastructure
Karyotyping
Kinetics
L Cells (Cell Line) - cytology
Mice
Molecular and cellular biology
Molecular genetics
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Summary:The dicentric and multicentric chromosomes in L cells and a brain tumor cell line of mouse display only one site of kinetochore formation associated with the 'active' centromere. The accessory or 'inactive' centromeres show premature separation. These cell lines were treated with 10(-6) M 5-bromodeoxyuridine (BrdUrd) followed by anti-BrdUrd antibody to study the pattern of replication of pericentric heterochromatin flanking the active vs inactive centromeres. Regardless of its quantity, heterochromatin around the inactive centromere replicates earlier than that associated with the active centromere. There appears to be a relationship between the timing of separation of a centromere and the timing of replication of pericentric heterochromatin. The premature replication of heterochromatin associated with an inactive centromere may be responsible for its premature separation and, hence, inactivity.
ISSN:0009-5915
1432-0886
DOI:10.1007/bf00286919