The e subunit gene of murine F1F0-ATP synthase: genomic sequence, chromosomal mapping, and diet regulation

Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence diffe...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-08, Vol.271 (34), p.20942-20948
Main Authors: Swartz, D.A. (University of Illinois, Urbana, IL.), Park, E.I, Visek, W.J, Kaput, J
Format: Article
Language:English
Subjects:
ACEITE DE MAIZ
ALIMENTACION
ALIMENTATION
Animals
ARN MENSAJERO
ARN MESSAGER
Base Sequence
CARTE GENETIQUE
Chromosome Mapping
COEUR
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CONSOMMATION ALIMENTAIRE
CONSUMO DE ALIMENTOS
CORAZON
CORPS GRAS
Diet
DIETA
Eating
EXPRESION GENICA
EXPRESSION DES GENES
FOIE
GENE
Gene Expression Regulation, Enzymologic
GENES
GENETICA
GENETIQUE
GRASAS
HIDROLASAS
HIGADO
HUILE DE MAIS
HYDROLASE
INANICION
INANITION
Liver - enzymology
MAPAS GENETICOS
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Myocardium - enzymology
Polymorphism, Restriction Fragment Length
Proton-Translocating ATPases - genetics
RATON
REGIME ALIMENTAIRE
RNA, Messenger - genetics
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SOURIS
Time Factors
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Summary:Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near DCMit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1.1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p 0.05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. The implications of the high degree of sequence similarity to the e subunit for rat and bovine F1F0-ATP synthase and its regulation by diet are discussed
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.34.20942