Toward Fully Automated High-Resolution Electron Tomography

Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT sy...

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Bibliographic Details
Published in:Journal of structural biology 1996-01, Vol.116 (1), p.181-189
Main Authors: Fung, Jennifer C., Liu, Weiping, de Ruijter, W.J., Chen, Hans, Abbey, Craig K., Sedat, John W., Agard, David A.
Format: Article
Language:English
Subjects:
Animals
Automation
Centrosome - ultrastructure
Computer Graphics
Computer Simulation
Microscopy, Electron
Models, Structural
Organelles - ultrastructure
Software
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Summary:Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT system, which automates both tomographic data collection and reconstruction.
ISSN:1047-8477
1095-8657
DOI:10.1006/jsbi.1996.0029